Abstract
The CRISPR-Cas system is involved in bacterial immunity, virulence, gene regulation, biofilm formation and sporulation. In Salmonella enterica serovar Typhi, this system consists of five transcriptional units including antisense RNAs. It was determined that these genetic elements are expressed in minimal medium and are up-regulated by pH. In addition, a transcriptional characterization of cas3 and ascse2-1 is included herein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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CRISPR-Associated Proteins / genetics*
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CRISPR-Cas Systems / genetics*
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Chloramphenicol O-Acetyltransferase / metabolism
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DNA Helicases / genetics*
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Electrophoretic Mobility Shift Assay
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Gene Expression Regulation, Bacterial / genetics*
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RNA, Antisense / genetics*
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Salmonella typhi / genetics*
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Transcription, Genetic / genetics
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Transcriptional Activation / genetics
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Up-Regulation / genetics
Substances
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CRISPR-Associated Proteins
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RNA, Antisense
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Chloramphenicol O-Acetyltransferase
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DNA Helicases