NMR-based metabolomics for the environmental assessment of Kaohsiung Harbor sediments exemplified by a marine amphipod (Hyalella azteca)

K H Chiu; C D Dong; C F Chen; M L Tsai; Y R Ju; T M Chen; C W Chen

doi:10.1016/j.marpolbul.2017.02.067

NMR-based metabolomics for the environmental assessment of Kaohsiung Harbor sediments exemplified by a marine amphipod (Hyalella azteca)

Mar Pollut Bull. 2017 Nov 30;124(2):714-724. doi: 10.1016/j.marpolbul.2017.02.067. Epub 2017 Mar 6.

Authors

K H Chiu¹, C D Dong², C F Chen³, M L Tsai⁴, Y R Ju³, T M Chen¹, C W Chen⁵

Affiliations

¹ Department and Graduate Institute of Aquaculture, National Kaohsiung Marine University, Kaohsiung, Taiwan.
² Department of Marine Environmental Engineering, National Kaohsiung Marine University, Kaohsiung, Taiwan; Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan.
³ Department of Marine Environmental Engineering, National Kaohsiung Marine University, Kaohsiung, Taiwan.
⁴ Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung, Taiwan.
⁵ Department of Marine Environmental Engineering, National Kaohsiung Marine University, Kaohsiung, Taiwan. Electronic address: cwchen@mail.nkmu.edu.tw.

PMID: 28267993
DOI: 10.1016/j.marpolbul.2017.02.067

Abstract

Inflow of wastewater from upstream causes a large flux of pollutants to enter Kaohsiung Harbor in Taiwan daily. To reveal the ecological risk posed by Kaohsiung Harbor sediments, an ecological metabolomic approach was employed to investigate environmental factors pertinent to the physiological regulation of the marine amphipod Hyalella azteca. The amphipods were exposed to sediments collected from different stream inlets of the Love River (LR), Canon River (CR), Jen-Gen River (JR), and Salt River (SR). Harbor entrance 1 (E1) was selected as a reference site. After 10-day exposure, metabolomic analysis of the Hyalella azteca revealed differences between two groups: {E1, LR, CR} and {JR, SR}. The metabolic pathways identified in the two groups of amphipods were significantly different. The results demonstrated that NMR-based metabolomics can be effectively used to characterize metabolic response related to sediment from polluted areas.

Keywords: Amphipod; Kaohsiung Harbor; Metabolomics; NMR; Risk assessment; Sediment.

MeSH terms

Amphipoda / metabolism*
Animals
Environmental Monitoring / methods*
Geologic Sediments / analysis
Magnetic Resonance Spectroscopy / methods*
Metabolomics / instrumentation
Metabolomics / methods*
Water Pollutants, Chemical / metabolism
Water Pollutants, Chemical / toxicity*

Substances

Water Pollutants, Chemical