Abstract
microRNAs are the post-transcriptional regulators implicated in the initiation and progression of various cancer types, including oral squamous cell carcinoma (OSCC). Here, we investigated the role of miR-377 in OSCC tumorigenesis. miR-377 expression was reduced in OSCC samples and cell line (UPCI-SCC-116), and was associated with patient survival. In vitro restoration of miR-377 repressed cell growth, induced apoptosis, and reduced cell migration. We identified HDAC9 as a target of miR-377 and found miR-377 to regulate HDAC9 and its pro-apoptotic target, NR4A1/Nur77. Our findings show that miR-377 targets HDAC9 pathway in OSCC, suggesting that miR-377-HDAC9 axis may provide a novel therapeutic target for OSCC therapy.
Keywords:
Histone deacetylase 9; Oral squamous cell carcinoma; microRNA-377.
MeSH terms
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3' Untranslated Regions
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Apoptosis
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Base Sequence
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Binding Sites
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Carcinoma, Squamous Cell / enzymology
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Carcinoma, Squamous Cell / genetics*
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Carcinoma, Squamous Cell / mortality
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Carcinoma, Squamous Cell / pathology
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Cell Line, Tumor
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Cell Movement
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Cell Proliferation
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Down-Regulation
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Enzyme Repression
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Gene Expression Regulation, Neoplastic*
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Histone Deacetylases
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Humans
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Kaplan-Meier Estimate
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MicroRNAs / genetics*
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MicroRNAs / metabolism
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Mouth Neoplasms / enzymology
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Mouth Neoplasms / genetics*
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Mouth Neoplasms / mortality
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Mouth Neoplasms / pathology
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Nuclear Receptor Subfamily 4, Group A, Member 1 / genetics
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Nuclear Receptor Subfamily 4, Group A, Member 1 / metabolism
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RNA Interference*
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Repressor Proteins
Substances
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3' Untranslated Regions
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MIRN377 microRNA, human
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MicroRNAs
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NR4A1 protein, human
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Nuclear Receptor Subfamily 4, Group A, Member 1
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Repressor Proteins
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HDAC9 protein, human
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Histone Deacetylases