Analyzing Cytokinin Responses During Plant-Nematode Interactions

Florian M W Grundler; Shahid Siddique

doi:10.1007/978-1-4939-6831-2_12

Analyzing Cytokinin Responses During Plant-Nematode Interactions

Methods Mol Biol. 2017:1569:151-158. doi: 10.1007/978-1-4939-6831-2_12.

Authors

Florian M W Grundler¹, Shahid Siddique²

Affiliations

¹ Department of Molecular Phytomedicine, Rheinische Friedrich-Wilhelms-University of Bonn, Karlrobert-Kreiten-Strasse 13, 53115, Bonn, Germany.
² Department of Molecular Phytomedicine, Rheinische Friedrich-Wilhelms-University of Bonn, Karlrobert-Kreiten-Strasse 13, 53115, Bonn, Germany. siddique@uni-bonn.de.

PMID: 28265995
DOI: 10.1007/978-1-4939-6831-2_12

Abstract

Cyst nematodes are obligate biotrophs that induce the formation of a hypertrophied and hypermetabolic syncytial-feeding site in roots of the host plants. Cytokinin signaling is activated at the site of infection and contributes significantly to the formation of syncytium. Here, we describe a protocol for visualizing cytokinin signaling activation in Arabidopsis upon infection with cyst nematode Heterodera schachtii using promoter reporter lines, TCSn:GFP and ARR5:GUS.

Keywords: ARR5:GUS; Cell cycle; Cytokinins; Giant cells; Nematode; Syncytium; TCSn:GFP.

MeSH terms

Animals
Arabidopsis / metabolism
Arabidopsis / parasitology
Cytokinins / metabolism*
Host-Parasite Interactions*
Plant Diseases / immunology
Plant Diseases / parasitology
Plant Growth Regulators / metabolism*
Plants / metabolism*
Plants / microbiology*
Plants, Genetically Modified
Tylenchoidea

Substances

Cytokinins
Plant Growth Regulators