Abstract
We identified the carbapenemase gene blaOXA-499, a variant of blaOXA-143, from a clinical isolate of Acinetobacter pittii for the first time. OXA-499 shared 93.1% amino acid identity with OXA-143, and the gene was located on the chromosome. By cloning the OXA-499-encoding gene into the pWH1266 vector and transforming it into susceptible Acinetobacter spp., we were able to show that OXA-499 confers resistance to carbapenems.
Keywords:
Acinetobacter pittii; OXA-143-like; OXA-499; carbapenemase resistant.
Copyright © 2017 American Society for Microbiology.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acinetobacter / drug effects
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Acinetobacter / genetics*
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Acinetobacter / isolation & purification
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Acinetobacter Infections / drug therapy*
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Acinetobacter Infections / microbiology
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Aged
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / genetics*
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Carbapenems / pharmacology*
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DNA, Bacterial / genetics
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Humans
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Male
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Microbial Sensitivity Tests
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Sequence Analysis, DNA
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beta-Lactam Resistance / genetics*
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beta-Lactamases / genetics*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Carbapenems
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DNA, Bacterial
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beta-Lactamases
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beta-lactamase OXA-143, Acinetobacter baumannii
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beta-lactamase OXA-499, Acinetobacter pittii
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carbapenemase