First Report of the Carbapenemase Gene blaOXA-499 in Acinetobacter pittii

Antimicrob Agents Chemother. 2017 Apr 24;61(5):e02676-16. doi: 10.1128/AAC.02676-16. Print 2017 May.

Abstract

We identified the carbapenemase gene blaOXA-499, a variant of blaOXA-143, from a clinical isolate of Acinetobacter pittii for the first time. OXA-499 shared 93.1% amino acid identity with OXA-143, and the gene was located on the chromosome. By cloning the OXA-499-encoding gene into the pWH1266 vector and transforming it into susceptible Acinetobacter spp., we were able to show that OXA-499 confers resistance to carbapenems.

Keywords: Acinetobacter pittii; OXA-143-like; OXA-499; carbapenemase resistant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter / drug effects
  • Acinetobacter / genetics*
  • Acinetobacter / isolation & purification
  • Acinetobacter Infections / drug therapy*
  • Acinetobacter Infections / microbiology
  • Aged
  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / genetics*
  • Carbapenems / pharmacology*
  • DNA, Bacterial / genetics
  • Humans
  • Male
  • Microbial Sensitivity Tests
  • Sequence Analysis, DNA
  • beta-Lactam Resistance / genetics*
  • beta-Lactamases / genetics*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Carbapenems
  • DNA, Bacterial
  • beta-Lactamases
  • beta-lactamase OXA-143, Acinetobacter baumannii
  • beta-lactamase OXA-499, Acinetobacter pittii
  • carbapenemase