Metabolite and gene expression responses in juvenile flounder Paralichthys olivaceus exposed to reduced salinities

Huifeng Wu; Jialin Liu; Zhen Lu; Lanlan Xu; Chenglong Ji; Qing Wang; Jianmin Zhao

doi:10.1016/j.fsi.2017.02.042

Metabolite and gene expression responses in juvenile flounder Paralichthys olivaceus exposed to reduced salinities

Fish Shellfish Immunol. 2017 Apr:63:417-423. doi: 10.1016/j.fsi.2017.02.042. Epub 2017 Feb 27.

Authors

Huifeng Wu¹, Jialin Liu², Zhen Lu², Lanlan Xu², Chenglong Ji³, Qing Wang³, Jianmin Zhao³

Affiliations

¹ Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, PR China. Electronic address: hfwu@yic.ac.cn.
² Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
³ Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, PR China.

PMID: 28245987
DOI: 10.1016/j.fsi.2017.02.042

Abstract

Seawater salinity is one of the most important changeable environmental factors influencing the behavior, survival, growth and production of marine organisms. In this work, metabolite and gene expression profiles were used to elucidate the biological effects of reduced salinities in juvenile flounder Paralichthys olivaceus. Metabolic profiling indicated that both reduced salinities (23.3‰ and 15.6‰) enhanced proteolysis and disturbed osmotic regulation and energy metabolism in juvenile flounder P. olivaceus. Furthermore, the low salinity (15.6‰) enhanced anaerobic metabolism indicated by the elevated lactate in flounder tissue extracts. Gene expression profiles exhibited that reduced salinities could induce immune stress and oxidative stress and disturb energy metabolism in juvenile flounder P. olivaceus. In addition, reduced salinities might promote the growth and gonadal differentiation in juvenile flounder P. olivaceus.

Keywords: Biological effects; Gene expression; Metabolite; Paralichthys olivaceus; Salinity.

MeSH terms

Animals
Energy Metabolism*
Flatfishes / genetics
Flatfishes / growth & development
Flatfishes / immunology
Flatfishes / physiology*
Gonads / growth & development
Immunity, Innate*
Metabolome*
Oxidative Stress*
Salinity*
Transcriptome*