Identification and Quantification of Human Brown Adipose Tissue

Maria Chondronikola; Craig Porter; John O Ogunbileje; Labros S Sidossis

doi:10.1007/978-1-4939-6820-6_16

Identification and Quantification of Human Brown Adipose Tissue

Methods Mol Biol. 2017:1566:159-176. doi: 10.1007/978-1-4939-6820-6_16.

Authors

Maria Chondronikola^{1

2}, Craig Porter^{3

4}, John O Ogunbileje^{3

4}, Labros S Sidossis^{5

6

7

8

9}

Affiliations

¹ Center for Human Nutrition, School of Medicine, Washington University in St. Louis, St. Louis, MO, USA.
² Harokopio University of Athens, Athens, Greece.
³ Department of Surgery, University of Texas Medical Branch at Galveston, Galveston, TX, USA.
⁴ Metabolism Unit, Shriners Hospitals for Children-Galveston, Galveston, TX, USA.
⁵ Harokopio University of Athens, Athens, Greece. lss133@rci.rutgers.edu.
⁶ Department of Surgery, University of Texas Medical Branch at Galveston, Galveston, TX, USA. lss133@rci.rutgers.edu.
⁷ Metabolism Unit, Shriners Hospitals for Children-Galveston, Galveston, TX, USA. lss133@rci.rutgers.edu.
⁸ Department of Medicine, Rutgers University, New Brunswick, NJ, 08901, USA. lss133@rci.rutgers.edu.
⁹ Department of Kinesiology and Health, Rutgers University, New Brunswick, NJ, 08901, USA. lss133@rci.rutgers.edu.

PMID: 28244050
DOI: 10.1007/978-1-4939-6820-6_16

Abstract

Brown adipose tissue (BAT) has attracted significant interest as a potential target tissue against obesity and its associated metabolic perturbations. The purpose of this chapter is to provide an overview of some of the methodological approaches that can be used to identify and quantify BAT in people. Specifically, we will provide a step-by-step description of the following procedures: quantification of BAT in vivo using positron emission tomography-computed tomography (PET/CT) with 2-deoxy-2-[¹⁸F]fluoroglucose (¹⁸F-FDG) as a tracer, mitochondrial respiration, and uncoupling protein 1 (UCP1) gene and protein expression.

Keywords: 2-deoxy-2-[18F]fluoroglucose; Biopsy; Brown adipose tissue; Positron emission tomography-computed tomography; Uncoupling protein 1.

MeSH terms

Adipose Tissue, Brown / diagnostic imaging*
Adipose Tissue, Brown / metabolism*
Biomarkers*
Cell Respiration
Fluorodeoxyglucose F18
Gene Expression
Humans
Mitochondria / genetics
Mitochondria / metabolism
Positron Emission Tomography Computed Tomography
Tomography, X-Ray Computed
Uncoupling Protein 1 / genetics
Uncoupling Protein 1 / metabolism

Substances

Biomarkers
Uncoupling Protein 1
Fluorodeoxyglucose F18