Reconstruction of Ligament and Tendon Defects Using Cell Technologies

R K Chailakhyan; A B Shekhter; S V Ivannikov; V I Tel'pukhov; D S Suslin; Yu V Gerasimov; A M Tonenkov; A G Grosheva; P V Panyushkin; I L Moskvina; N N Vorob'eva; V N Bagratashvili

doi:10.1007/s10517-017-3660-y

Reconstruction of Ligament and Tendon Defects Using Cell Technologies

Bull Exp Biol Med. 2017 Feb;162(4):563-568. doi: 10.1007/s10517-017-3660-y. Epub 2017 Feb 28.

Authors

Affiliations

¹ N. F. Gamaleya Research Centre of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia. rubenchail@yandex.ru.
² I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
³ N. F. Gamaleya Research Centre of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
⁴ Institute of Photonics Technologies, Russian Academy of Sciences, Federal Research Center "Crystallography and Photonics", Russian Academy of Sciences, Moscow, Russia.

PMID: 28243921
DOI: 10.1007/s10517-017-3660-y

Abstract

We studied the possibility of restoring the integrity of the Achilles tendon in rabbits using autologous multipotent stromal cells. Collagen or gelatin sponges populated with cells were placed in a resorbable Vicryl mesh tube and this tissue-engineered construct was introduced into a defect of the middle part of the Achilles tendon. In 4 months, histological analysis showed complete regeneration of the tendon with the formation of parallel collagen fibers, spindle-shaped tenocytes, and newly formed vessels.

Keywords: ligaments and tendons; multipotent stromal cells; regeneration; tissue-engineered construct.

MeSH terms

Achilles Tendon / injuries
Achilles Tendon / surgery*
Achilles Tendon / ultrastructure
Animals
Biomechanical Phenomena
Collagen / chemistry
Gelatin / chemistry
Ligaments / injuries
Ligaments / surgery*
Ligaments / ultrastructure
Male
Mesenchymal Stem Cell Transplantation*
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / physiology
Plastic Surgery Procedures / instrumentation
Plastic Surgery Procedures / methods*
Polyglactin 910 / chemistry
Rabbits
Tenocytes / cytology
Tenocytes / physiology
Tensile Strength
Tissue Engineering
Tissue Scaffolds / chemistry
Transplantation, Autologous
Wound Healing / physiology*

Substances

Polyglactin 910
Gelatin
Collagen