Molecular Epidemiology of Colistin-Resistant, Carbapenemase-Producing Klebsiella pneumoniae in Serbia from 2013 to 2016

Katarina Novović; Anika Trudić; Snežana Brkić; Zorica Vasiljević; Milan Kojić; Deana Medić; Ivana Ćirković; Branko Jovčić

doi:10.1128/AAC.02550-16

Molecular Epidemiology of Colistin-Resistant, Carbapenemase-Producing Klebsiella pneumoniae in Serbia from 2013 to 2016

Antimicrob Agents Chemother. 2017 Apr 24;61(5):e02550-16. doi: 10.1128/AAC.02550-16. Print 2017 May.

Authors

Katarina Novović¹, Anika Trudić^{2

3}, Snežana Brkić⁴, Zorica Vasiljević⁵, Milan Kojić¹, Deana Medić^{2

6}, Ivana Ćirković⁷, Branko Jovčić^{8

9}

Affiliations

¹ Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.
² Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia.
³ Institute for Pulmonary Diseases of Vojvodina, Sremska Kamenica, Serbia.
⁴ Institute for Laboratory Diagnostics Konzilijum, Belgrade, Serbia.
⁵ Department of Clinical Microbiology, Mother and Child Health Care Institute of Serbia Dr. Vukan Cupic, Belgrade, Serbia.
⁶ Institute of Public Health of Vojvodina, Novi Sad, Serbia.
⁷ Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
⁸ Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia bjovcic@bio.bg.ac.rs.
⁹ Faculty of Biology, University of Belgrade, Belgrade, Serbia.

Abstract

Twenty-seven colistin-resistant, carbapenemase-producing Klebsiella pneumoniae isolates were identified from hospitals in Serbia. All isolates were bla_CTX-M-15 positive; ST101, ST888, ST437, ST336, and ST307 were bla_OXA-48 positive; and ST340 was bla_NDM-1 positive. ST307 had an insertion, and ST336 had a premature stop codon in the mgrB gene. Amino acid substitutions were detected in PmrAB of isolates ST101, ST888, ST336, and ST307. The mcr-1 and mcr-2 were not detected. An increase in phoP, phoQ, and pmrK gene transcription was detected for all sequence types.

Keywords: Klebsiella pneumoniae; carbapenem resistance; colistin resistance; molecular epidemiology.

MeSH terms

Bacterial Proteins / biosynthesis*
Child, Preschool
Colistin / therapeutic use*
Drug Resistance, Bacterial / genetics
Humans
Klebsiella Infections / drug therapy
Klebsiella Infections / epidemiology*
Klebsiella Infections / microbiology
Klebsiella pneumoniae / drug effects*
Klebsiella pneumoniae / genetics
Klebsiella pneumoniae / isolation & purification
Microbial Sensitivity Tests
Molecular Epidemiology
Serbia / epidemiology
beta-Lactamases / biosynthesis*

Substances

Bacterial Proteins
beta-lactamase CTX-M-15
beta-Lactamases
beta-lactamase NDM-1
carbapenemase
oxacillinase
Colistin