Objective: To investigate the changes in protein expression in patients with 1-bromopropane (1-BP) poisoning using high-throughput proteomic technique and to screen out protein markers. Methods: Serum samples were collected from 3 patients with 1-BP poisoning and 15 controls. The label-free proteomic tech-nique was used for the quantitation and identification of proteins expressed in these samples, and the results were compared between the patients with 1-BP poisoning and the control population. The bioinformatics tools were used to analyze the function of differentially expressed proteins. Results: Compared with the control popula-tion, the patients with 1-BP poisoning had >2-fold upregulation of 38 proteins and >2-fold downregulation of 68 proteins. The differentially expressed proteins were mainly involved in immune response, signal transduction, and stress response. Conclusion: The proteins screened out may be potential protein markers for 1-BP poison-ing, which provides reliable and precise methods and thoughts for the diagnosis of 1-BP poisoning.
目的：利用高通量蛋白质组技术检测分析1-溴丙烷（1-bromopropane ：1-BP）中毒患者的蛋白质表达变化，筛选蛋白标志物。 方法：收集1-BP中毒患者（3例）和对照（15例）血清样本，采用Label free蛋白质谱分析技术，对1-BP和正常样本中表达的蛋白进行定量和鉴定，并与对照人群进行比较，利用生物信息学工具分析差异表达蛋白的功能。 结果：与对照人群比较，1-BP中毒患者血清中检测到38个蛋白质上调2倍以上，68个蛋白质下调2倍以上。这些差异表达的蛋白质主要参与免疫反应、信号转导、应激反应等。 结论：筛选的蛋白质是潜在的1-BP中毒蛋白标志，为1-BP中毒诊断提供了更可靠和精确的方法及思路。.
Keywords: 1-Bromopropane; Poisoning; Protein.