Expression of Surface Molecules in Human Mesenchymal Stromal Cells Co-Cultured with Nucleated Umbilical Cord Blood Cells

Bull Exp Biol Med. 2017 Feb;162(4):578-582. doi: 10.1007/s10517-017-3662-9. Epub 2017 Feb 27.

Abstract

We studied the expression of different classes of surface molecules (CD13, CD29, CD40, CD44, CD54, CD71, CD73, CD80, CD86, CD90, CD105, CD106, CD146, HLA-I, and HLA-DR) in mesenchymal stromal cells from human umbilical cord and bone marrow during co-culturing with nucleated umbilical cord blood cells. Expression of the majority of surface markers in both types of mesenchymal stromal cells was stable and did not depend on the presence of the blood cells. Significant differences were found only for cell adhesion molecules CD54 (ICAM-1) and CD106 (VCAM-1) responsible for direct cell-cell contacts with leukocytes and only for bone marrow derived cells.

Keywords: co-culture; differentiation clusters; flow cytometry; mesenchymal stromal cells; umbilical cord blood.

MeSH terms

  • Antigens, CD / genetics
  • Antigens, CD / metabolism
  • Blood Cells / cytology
  • Blood Cells / metabolism*
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / metabolism*
  • Cell Adhesion
  • Cell Differentiation
  • Cell Nucleus / ultrastructure
  • Cell Separation / methods
  • Coculture Techniques
  • Fetal Blood / cytology
  • Fetal Blood / metabolism*
  • Flow Cytometry
  • Gene Expression*
  • HLA-DR Antigens / genetics
  • HLA-DR Antigens / metabolism
  • Humans
  • Immunophenotyping
  • Intercellular Adhesion Molecule-1 / genetics
  • Intercellular Adhesion Molecule-1 / metabolism
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism*
  • Primary Cell Culture
  • Vascular Cell Adhesion Molecule-1 / genetics
  • Vascular Cell Adhesion Molecule-1 / metabolism

Substances

  • Antigens, CD
  • HLA-DR Antigens
  • Vascular Cell Adhesion Molecule-1
  • Intercellular Adhesion Molecule-1