Synthesis and application of in-situ molecularly imprinted silica monolithic in pipette-tip solid-phase microextraction for the separation and determination of gallic acid in orange juice samples

Maryam Arabi; Mehrorang Ghaedi; Abbas Ostovan

doi:10.1016/j.jchromb.2017.02.016

Synthesis and application of in-situ molecularly imprinted silica monolithic in pipette-tip solid-phase microextraction for the separation and determination of gallic acid in orange juice samples

J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Mar 24:1048:102-110. doi: 10.1016/j.jchromb.2017.02.016. Epub 2017 Feb 16.

Authors

Maryam Arabi¹, Mehrorang Ghaedi², Abbas Ostovan³

Affiliations

¹ Chemistry Department, Yasouj University, Yasouj, 75914-35, Iran.
² Chemistry Department, Yasouj University, Yasouj, 75914-35, Iran. Electronic address: m_ghaedi@mail.yu.ac.ir.
³ Department of Chemistry, Kerman Branch, Islamic Azad University, Kerman, Iran.

PMID: 28235747
DOI: 10.1016/j.jchromb.2017.02.016

Abstract

A novel strategy was presented for the synthesis and application of functionalized silica monolithic as artificial receptor of gallic acid at micro-pipette tip. A sol-gel process was used to prepare the sorbent. In this in-situ polymerization reaction, tetraethyl orthosilicate (TEOS), 3-aminopropyl trimethoxysilane (APTMS), gallic acid and thiourea were used, respectively, as cross-linker, functionalized monomer, template and precursor to make crack-free and non-fragile structure. Such durable and inexpensive in-situ monolithic was successfully employed as useful tool for highly efficient extraction of gallic acid from orange juice samples. The effective parameters in extraction recovery were investigated and optimum conditions were obtained using experimental design methodology. Applying HPLC-UV for separation quantification at optimal conditions, the gallic acid was efficiently extracted without significant matrix interference. Good linearity for gallic acid in the range of 0.02-5.0mgL^-1 with correlation coefficients of R²>0.999 revealed well applicability of the method for trace analysis.

Keywords: Experimental design; Gallic acid; HPLC-UV; Molecularly imprinted; Monolithic; Pipette-tip solid phase microextraction.

Publication types

Evaluation Study

MeSH terms

Chromatography, High Pressure Liquid / methods*
Citrus sinensis / chemistry
Food Analysis / methods
Fruit and Vegetable Juices / analysis*
Gallic Acid / analysis
Gallic Acid / isolation & purification*
Limit of Detection
Molecular Imprinting / methods*
Polymerization
Propylamines / chemistry
Silanes / chemistry
Silicon Dioxide / chemistry*
Solid Phase Microextraction / methods*

Substances

Propylamines
Silanes
3-aminopropyltrimethoxysilane
tetraethoxysilane
Gallic Acid
Silicon Dioxide