Nanostructured polysaccharidic microcapsules for intracellular release of cisplatin

Abstract

Carbohydrate polimeric microcapsules were assembled using a LbL approach onto a CaCO₃ core. The microcapsules were used to delivery the anticancer drug cisplatin into HeLa and MCF-7 cancer cell lines. Drug encapsulation, measured by ICP spectroscopy, was around 50% of the charging solution. Fluorimetric measurements showed an efficient cellular uptake of polysacchardic microcapsules in both cell lines. The drug-loaded capsules demonstrated a better efficiency against cell viability than the free drug. Specifically, the amount of platinum reaching genomic DNA was measured, showing that encapsulation improves the nuclear delivery of the drug for both cell lines.

Keywords: Chitosan; Cisplatin; LbL; Pectin; Polysaccharidic microcapsules.