Direct and label-free influenza virus detection based on multisite binding to sialic acid receptors

Yukichi Horiguchi; Tatsuro Goda; Akira Matsumoto; Hiroaki Takeuchi; Shoji Yamaoka; Yuji Miyahara

doi:10.1016/j.bios.2017.02.023

Direct and label-free influenza virus detection based on multisite binding to sialic acid receptors

Biosens Bioelectron. 2017 Jun 15:92:234-240. doi: 10.1016/j.bios.2017.02.023. Epub 2017 Feb 16.

Authors

Yukichi Horiguchi¹, Tatsuro Goda¹, Akira Matsumoto¹, Hiroaki Takeuchi², Shoji Yamaoka², Yuji Miyahara³

Affiliations

¹ Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University (TMDU), 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan.
² Department of Molecular Virology, Graduate School of Medical and Dental Sciences, TMDU, 1-5-45 Yushima, Bunkyo, Tokyo 113-8510, Japan.
³ Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University (TMDU), 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan. Electronic address: miyahara.bsr@tmd.ac.jp.

Abstract

A system to discriminate human or avian influenza A remains a highly sought-after tool for prevention of influenza pandemics in humans. Selective binding of the influenza A viral hemagglutinin (HA) to specific sialic acid (SA) receptors (Neu5Acα(2-6)Gal in humans, Neu5Acα(2-3)Gal in birds) is determined by the genotype of the HA and neuraminidase (NA) segments, making it one of the key characteristics that distinguishes human or avian influenza A virus. Here we demonstrate the direct detection of whole H1N1 influenza A virus using 6'-sialyllactose (Neu5Acα(2-6)Galβ(1-4)Glc, 6SL)-immobilized gold electrodes as biosensing surfaces. The sensitivity was higher than that of conventional immunochromatographic technique (ICT) for influenza virus and not restricted by genetic drift. The label-free detection technology via direct attachment of a whole virus using a chemically modified electrode is a promising means to provide a simple and rapid diagnostic system for viral infections.

Keywords: Biointerface; Biosensor; Influrenza virus; Label-free; Molecular recognition.

Publication types

Evaluation Study

MeSH terms

Biosensing Techniques / methods*
Chromatography, Affinity
Electrochemical Techniques / methods
Electrodes
Gold / chemistry
Humans
Influenza A Virus, H1N1 Subtype / isolation & purification*
Influenza, Human / diagnosis
Influenza, Human / virology*
Lactose / analogs & derivatives*
Lactose / chemistry
Limit of Detection
Quartz Crystal Microbalance Techniques / methods

Substances

6'-sialyllactose
Gold
Lactose