Objective: To investigate effects of retinol on the expressions of epidermal growth factor (EGF), stem cell factor (SCF), colony-stimulating factor 1 (CSF1) and leukemia inhibitory factor (LIF) in cultured human umbilical-derived mesenchymal stem cells (UCMSCs).
Methods: Human UCMSCs were isolated from human umbilical cord and identified for immunophenotypes. The cells were then cultured in DMEM/F12 media supplemented with 12% fetal bovine serum (FBS), 12% FBS+1 µmol/L retinol, 15% knockout serum replacement (KSR) and 15% KSR+ 1 µmol/L retinol. The expressions of the cytokines EGF, SCF, CSF1 and LIF in the cells were detected using RT-PCR and ELISA.
Results: The isolated cells exhibited characteristic immunophenotypes of human UCMSCs and expressed EGF, CSF1 and SCF at both mRNA and protein levels but not LIF protein. Retinol (1 µmol/L) significantly promoted the expressions of SCF and CSF1 at both mRNA and protein levels but did not result in changes of EGF and LIF expressions in human UCMSCs.
Conclusion: Retinol at the concentration of 1 µmol/L can promote expression of SCF and CSF1 in human UCMSCs in vitro.
目的: 研究视黄醇（维生素A）对人脐带间充质干细胞表达表皮生长因子（EGF）、干细胞因子（SCF）、集落刺激因子1（CSF1）和白血病抑制因子（LIF）的影响。
方法: 分离鉴定人脐带间充质干细胞，待细胞生长稳定后分为4 组，分别用含12%FBS、12% FBS+1 μmol/L视黄醇、15%血清替代品（KSR）和15% KSR+1 μmol/L视黄醇的DMEM/F12培养液培养，3 d后收集细胞及培养液上清，用RT-PCR和ELISA分析视黄醇对人脐带间充质干细胞生成细胞因子EGF、SCF、CSF1和LIF的影响。
结果: 分离所得细胞具备人脐带间充质干细胞免疫表型特征，细胞因子EGF、SCF和CSF1在mRNA和蛋白质水平均表达，而LIF只在mRNA水平表达；添加视黄醇（1 μmol/L）后，SCF和CSF1在mRNA、蛋白质水平均显著增加（P＜0.05），而EGF和LIF变化不显著（P≥0.05）。
结论: 视黄醇（1 mol/L）可促进体外培养的人脐带间充质干细胞生成细胞因子SCF和CSF1。