Draft genome sequence of reduced teicoplanin-susceptible and vancomycin-heteroresistant methicillin-resistant Staphylococcus aureus from sepsis cases

J Glob Antimicrob Resist. 2017 Mar:8:169-171. doi: 10.1016/j.jgar.2016.12.008. Epub 2017 Feb 23.

Abstract

Here we report the whole-genome shotgun sequence of six methicillin-resistant Staphylococcus aureus (MRSA) showing reduced susceptibility to both vancomycin and teicoplanin. The typical Indian community-acquired MRSA (CA-MRSA) clone ST772-MRSA-V-t657 was the most common genotype (3/6; 50%), followed by ST672-MRSA-IV (2/6; 33%) and ST22-MRSA-IV (1/6; 17%). All strains harboured a mutation in the tcaRAB operon, vraSR, graSR and/or rpoB genes, which are frequently mutated determinants in a heteroresistant vancomycin-intermediate S. aureus (hVISA) phenotype.

Keywords: MRSA; hVISA; tcaA.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Computational Biology
  • Genes, Bacterial
  • Genome, Bacterial*
  • Humans
  • Methicillin-Resistant Staphylococcus aureus / drug effects
  • Methicillin-Resistant Staphylococcus aureus / genetics*
  • Methicillin-Resistant Staphylococcus aureus / isolation & purification
  • Sepsis / microbiology*
  • Sequence Analysis, DNA*
  • Staphylococcal Infections / microbiology*
  • Teicoplanin / pharmacology
  • Vancomycin / pharmacology
  • Vancomycin Resistance*
  • Whole Genome Sequencing

Substances

  • Anti-Bacterial Agents
  • Teicoplanin
  • Vancomycin