Manipulating Cholesterol Status Within Cells

Winnie Luu; Ingrid C Gelissen; Andrew J Brown

doi:10.1007/978-1-4939-6875-6_4

Manipulating Cholesterol Status Within Cells

Methods Mol Biol. 2017:1583:41-52. doi: 10.1007/978-1-4939-6875-6_4.

Authors

Winnie Luu¹, Ingrid C Gelissen², Andrew J Brown³

Affiliations

¹ School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Biological Sciences Building D26, Sydney, NSW, 2052, Australia.
² Faculty of Pharmacy, The University of Sydney, Sydney, NSW, 2006, Australia.
³ School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Biological Sciences Building D26, Sydney, NSW, 2052, Australia. aj.brown@unsw.edu.au.

PMID: 28205165
DOI: 10.1007/978-1-4939-6875-6_4

Abstract

Cellular cholesterol levels are intricately controlled to maintain homeostasis. Here, we describe ways in which cellular cholesterol status can be manipulated for the study of cholesterol homeostasis, including sterol starvation (by culturing cells in lipoprotein-deficient serum and pretreating/treating with the cholesterol-lowering drug, statin) and sterol enrichment (using cholesterol complexed to cyclodextrin, and low-density lipoprotein). We also describe how to prepare lipoprotein-deficient serum and complex cholesterol to cyclodextrin.

Keywords: Chinese hamster ovary cells; Cholesterol; LDL; Lipoprotein-deficient serum; Statins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Cholesterol / metabolism*
Cyclodextrins / chemistry
Cyclodextrins / pharmacology*
Homeostasis / drug effects*
Humans
Hydroxymethylglutaryl-CoA Reductase Inhibitors / chemistry
Hydroxymethylglutaryl-CoA Reductase Inhibitors / pharmacology*
Lipoproteins, LDL / chemistry
Lipoproteins, LDL / pharmacology*

Substances

Cyclodextrins
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Lipoproteins, LDL
Cholesterol