Rat transformation-associated proteins (TAP) induced by Moloney murine sarcoma virus interact with specific receptors on normal rat kidney cells

Biochem Biophys Res Commun. 1987 Aug 31;147(1):513-8. doi: 10.1016/s0006-291x(87)80151-1.

Abstract

In this report, data are presented to show that transformation-associated proteins (TAP) secreted from the transformed 6M2 cells have mitogenic activities in the stimulation of DNA synthesis and proliferation of normal rat kidney (NRK-2) cells and of nonpermissively grown 6M2 cells. TAP also bound specifically to NRK-2 cells with a binding dissociation constant (Kd) of 1.4 pM. Approximately 2 X 10(5) binding sites per cell were found. Therefore, TAP may represent a set of virally-induced growth stimulatory factors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Cell Division
  • Cell Transformation, Neoplastic / physiopathology*
  • Growth Substances / physiology*
  • Insulin / pharmacology
  • Kidney / metabolism*
  • Mitogens*
  • Molecular Weight
  • Moloney murine sarcoma virus
  • Peptides / physiology*
  • Rats
  • Receptors, Cell Surface / metabolism*
  • Transforming Growth Factors

Substances

  • Antibodies, Monoclonal
  • Growth Substances
  • Insulin
  • Mitogens
  • Peptides
  • Receptors, Cell Surface
  • Transforming Growth Factors