Abstract
In this report, data are presented to show that transformation-associated proteins (TAP) secreted from the transformed 6M2 cells have mitogenic activities in the stimulation of DNA synthesis and proliferation of normal rat kidney (NRK-2) cells and of nonpermissively grown 6M2 cells. TAP also bound specifically to NRK-2 cells with a binding dissociation constant (Kd) of 1.4 pM. Approximately 2 X 10(5) binding sites per cell were found. Therefore, TAP may represent a set of virally-induced growth stimulatory factors.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal
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Cell Division
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Cell Transformation, Neoplastic / physiopathology*
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Growth Substances / physiology*
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Insulin / pharmacology
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Kidney / metabolism*
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Mitogens*
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Molecular Weight
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Moloney murine sarcoma virus
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Peptides / physiology*
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Rats
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Receptors, Cell Surface / metabolism*
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Transforming Growth Factors
Substances
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Antibodies, Monoclonal
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Growth Substances
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Insulin
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Mitogens
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Peptides
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Receptors, Cell Surface
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Transforming Growth Factors