Potential toxicity of ionic liquid ([C12mim]BF4) on the growth and biochemical characteristics of a marine diatom Phaeodactylum tricornutum

Abstract

Recently, some researchers have pointed out that the threats of ionic liquids (ILs) to aquatic environment cannot be ignored. Thus, this study investigated the potential toxicity of 1-dodecyl-3-methylimidazolium tetrafluoroborate ([C₁₂mim]BF₄) on a marine diatom Phaeodactylum tricornutum at population, biochemical and physiological levels using 96h growth tests with a batch-culture system. Results showed that [C₁₂mim]BF₄ was very stable in aquatic environment during 96h of exposure. The growth of P. tricornutum was significantly inhibited by [C₁₂mim]BF₄ with 24, 48, 72 and 96h EC₅₀ values of 0.63, 0.61, 0.68 and 0.72mgL^-1, respectively. Although there were no significant differences between the controls and treatments with 0.1 and 0.5mgL^-1 [C₁₂mim]BF₄, the effective quantum yields (Φ_PSII) of the diatom in 1, 2.5, 5 and 10mgL^-1 [C₁₂mim]BF₄ treatments were 61.48, 17.04, 2.96 and 0.74% of that in the controls at 96h of exposure, respectively. Chl a content of the diatom was decreased by 34.86, 47.79, 49.81, 59.21, 79.82 and 86.98% compared with that of the controls at 96h of exposure in 0.1, 0.5, 1, 2.5, 5 and 10mgL^-1 [C₁₂mim]BF₄ treatments, respectively. Relative to the controls, soluble sugar content, reactive oxygen species (ROS) level, malondialdehyde (MDA) content, superoxide dismutase (SOD) and peroxidase (POD) activities of the diatom increased with increasing [C₁₂mim]BF₄ concentrations at 96h of exposure, and reached their maxima (1.46μg10⁶cell^-1, 7.48FU10⁷cell^-1, 3.35nmol10⁸cell^-1, 33.41 and 7.23Umg^-1 proteins, respectively) in 5mgL^-1 [C₁₂mim]BF₄ treatments. While the maximum soluble protein content (1.56μg10⁶cell^-1) of the diatom was obtained in 0.5mgL^-1 [C₁₂mim]BF₄ treatments, and then decreased with increasing [C₁₂mim]BF₄ concentrations from 0.5 to 10mgL^-1. These findings provide strong evidence for the potential toxicity of ILs to marine diatoms.

Keywords: 1-dodecyl-3-methylimidazolium tetrafluoroborate; Antioxidant enzymes; Ionic liquid; Phaeodactylum tricornutum; Photosynthetic activity.