In Vivo Gold Complex Catalysis within Live Mice

Angew Chem Int Ed Engl. 2017 Mar 20;56(13):3579-3584. doi: 10.1002/anie.201610273. Epub 2017 Feb 15.

Abstract

Metal complex catalysis within biological systems is largely limited to cell and bacterial systems. In this work, a glycoalbumin-AuIII complex was designed and developed that enables organ-specific, localized propargyl ester amidation with nearby proteins within live mice. The targeted reactivity can be imaged through the use of Cy7.5- and TAMRA-linked propargyl ester based fluorescent probes. This targeting system could enable the exploitation of other metal catalysis strategies for biomedical and clinical applications.

Keywords: amide bond formation; fluorescent labeling; glycoalbumin; gold catalysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Catalysis
  • Coordination Complexes / chemistry*
  • Coordination Complexes / pharmacokinetics
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / pharmacokinetics
  • Glycated Serum Albumin
  • Glycation End Products, Advanced
  • Gold / chemistry*
  • Gold / pharmacokinetics
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Optical Imaging / methods
  • Serum Albumin / chemistry*
  • Serum Albumin / pharmacokinetics
  • Tissue Distribution

Substances

  • Coordination Complexes
  • Fluorescent Dyes
  • Glycation End Products, Advanced
  • Serum Albumin
  • Gold
  • Glycated Serum Albumin