Abstract
The SmeDEF pump of Stenotrophomonas maltophilia is negatively regulated by SmeT. In this study, strains KJΔT (smeT deletion mutant) and KJT-Dm (mutant with a defective SmeT-binding site) showed increased resistance to chloramphenicol/nalidixic acid/macrolides and susceptibility to aminoglycoside. Overexpression of the SmeDEF pump, in either KJΔT or KJT-Dm, downregulated smeYZ expression, which is responsible for the reduced aminoglycoside resistance. Furthermore, the SmeRySy two-component regulatory system was downregulated in response to SmeDEF overexpression, which supports its involvement in the regulatory circuit.
Keywords:
antibiotic resistance; bacteria; efflux pump; two-component regulatory system.
Copyright © 2017 American Society for Microbiology.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amikacin / pharmacology
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Aminoglycosides / pharmacology*
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / biosynthesis*
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Bacterial Proteins / genetics
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Chloramphenicol / pharmacology
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Ciprofloxacin / pharmacology
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Drug Resistance, Multiple, Bacterial / genetics
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Erythromycin / pharmacology
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Gentamicins / pharmacology
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Kanamycin / pharmacology
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Membrane Transport Proteins / biosynthesis*
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Membrane Transport Proteins / genetics
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Stenotrophomonas maltophilia / drug effects*
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Stenotrophomonas maltophilia / genetics*
Substances
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Aminoglycosides
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Anti-Bacterial Agents
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Bacterial Proteins
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Gentamicins
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Membrane Transport Proteins
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SmeD protein, Stenotrophomonas maltophilia
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SmeE protein, Stenotrophomonas maltophilia
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SmeF protein, Stenotrophomonas maltophilia
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Kanamycin
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Ciprofloxacin
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Erythromycin
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Chloramphenicol
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Amikacin