Direct extraction of tetracyclines from bovine milk using restricted access carbon nanotubes in a column switching liquid chromatography system

Henrique Dipe de Faria; Mariana Azevedo Rosa; Alberto Thalison Silveira; Eduardo Costa Figueiredo

doi:10.1016/j.foodchem.2017.01.004

Direct extraction of tetracyclines from bovine milk using restricted access carbon nanotubes in a column switching liquid chromatography system

Food Chem. 2017 Jun 15:225:98-106. doi: 10.1016/j.foodchem.2017.01.004. Epub 2017 Jan 3.

Authors

Henrique Dipe de Faria¹, Mariana Azevedo Rosa¹, Alberto Thalison Silveira¹, Eduardo Costa Figueiredo²

Affiliations

¹ Laboratory of Toxicant and Drug Analyses, Gabriel Monteiro da Silva St. 700, Federal University of Alfenas - Unifal-MG, 37130-000, Alfenas, MG, Brazil.
² Laboratory of Toxicant and Drug Analyses, Gabriel Monteiro da Silva St. 700, Federal University of Alfenas - Unifal-MG, 37130-000, Alfenas, MG, Brazil. Electronic address: eduardocfig@yahoo.com.br.

PMID: 28193438
DOI: 10.1016/j.foodchem.2017.01.004

Abstract

This paper describes, for the first time, the use of restricted access carbon nanotubes (RACNTs) in the analysis of tetracyclines from milk samples, in a multidimensional liquid chromatographic system. Milk samples were initially acidified and centrifuged, and then the supernatant was directly analyzed in a column switching system in backflush configuration employing an extraction column of RACNTs. The sorbent was able to exclude all the remained proteins in less than 2.0min. The method was linear from 50 to 200μgL^-1 and the coefficients of determination (r²) were 0.997, 0.992, 0.994 and 0.998 for oxytetracycline (OXI), tetracycline (TC), chlortetracycline (CTC) and doxycycline (DOX), respectively. The analytical range included the maximum residue limits established by the regulatory agency.

Keywords: Carbon nanotubes; Chlortetracycline (PubChem CID: 54675777); Column switching; Doxycycline (PubChem CID: 54671203); Liquid chromatography; Milk; Oxytetracycline (PubChem CID: 54675779); Restricted access carbon nanotubes; Restricted access materials; Tetracycline (PubChem CID: 54675776); Tetracyclines.

MeSH terms

Animals
Chromatography, Liquid*
Milk / chemistry
Nanotubes, Carbon / chemistry*
Tetracyclines / analysis
Tetracyclines / chemistry
Tetracyclines / isolation & purification*

Substances

Nanotubes, Carbon
Tetracyclines