4-hydroxybenzaldehyde-chitooligomers suppresses H2O2-induced oxidative damage in microglia BV-2 cells

Sea-Hun Oh; BoMi Ryu; Dai-Hung Ngo; Won-Suk Kim; Dong Gyu Kim; Se-Kwon Kim

doi:10.1016/j.carres.2017.01.007

4-hydroxybenzaldehyde-chitooligomers suppresses H₂O₂-induced oxidative damage in microglia BV-2 cells

Carbohydr Res. 2017 Feb 22:440-441:32-37. doi: 10.1016/j.carres.2017.01.007. Epub 2017 Jan 16.

Authors

Sea-Hun Oh¹, BoMi Ryu², Dai-Hung Ngo³, Won-Suk Kim⁴, Dong Gyu Kim⁵, Se-Kwon Kim⁶

Affiliations

¹ Marine Bioprocess Research Center, Pukyong National University, Busan, 608-739, Republic of Korea.
² School of Pharmacy, The University of Queensland, Brisbane, QLD, 4072, Australia.
³ Faculty of Resources and Environment, Thu Dau Mot University, Binh Duong, Viet Nam.
⁴ Major in Pharmaceutical Engineering Division of Bio-Industry, Silla University, Busan, Republic of Korea.
⁵ Specialized Graduate School Science and Technology Convergence, Department of Marine Bio Convergence Science, Pukyong National University, Busan, 608-737, Republic of Korea. Electronic address: dgkim@pknu.ac.kr.
⁶ Marine Bioprocess Research Center, Pukyong National University, Busan, 608-739, Republic of Korea; Specialized Graduate School Science and Technology Convergence, Department of Marine Bio Convergence Science, Pukyong National University, Busan, 608-737, Republic of Korea. Electronic address: sknkim@pknu.ac.kr.

PMID: 28192685
DOI: 10.1016/j.carres.2017.01.007

Abstract

Positive charges of chitooligomer (COS) enable COS to interact with negatively charged anionic groups on the cell surface resulting in an improvement in the biological activity of COS and its derivatives. In this study, 4-hydroxybenzaldehyde-COS (HB-COS) was synthesized and investigated for its abilities against H₂O₂-induced oxidative stress in microglia BV-2 cells. Under oxidative stress, HB-COS significantly attenuated reactive oxygen species (ROS) generation and DNA oxidation, and upregulated the protein levels of antioxidative enzymes. HB-COS is therefore proposed as a potential protective agent against neuronal damage.

Keywords: 4-hydroxybenzaldehyde-COS (HB-COS); Microglia BV-2 cells; Oxidative stress.

MeSH terms

Animals
Antioxidants / chemical synthesis
Antioxidants / pharmacology*
Benzaldehydes / chemistry
Benzaldehydes / pharmacology*
Catalase / metabolism
Cell Line, Transformed
Cell Survival / drug effects
Chitin / analogs & derivatives*
Chitin / chemical synthesis
Chitin / pharmacology
Chitosan
Fluoresceins / chemistry
Fluorescent Dyes / chemistry
Glutathione / metabolism
Glutathione Peroxidase / metabolism
Glutathione Reductase / metabolism
Hydrogen Peroxide / antagonists & inhibitors*
Hydrogen Peroxide / pharmacology
Mice
Microglia / cytology
Microglia / drug effects*
Microglia / metabolism
Neuroprotective Agents / chemical synthesis
Neuroprotective Agents / pharmacology*
Oligosaccharides
Oxidation-Reduction
Oxidative Stress
Reactive Oxygen Species / antagonists & inhibitors
Reactive Oxygen Species / metabolism
Static Electricity
Superoxide Dismutase / metabolism

Substances

Antioxidants
Benzaldehydes
Fluoresceins
Fluorescent Dyes
Neuroprotective Agents
Oligosaccharides
Reactive Oxygen Species
oligochitosan
Chitin
diacetyldichlorofluorescein
Chitosan
Hydrogen Peroxide
Catalase
Glutathione Peroxidase
Superoxide Dismutase
Glutathione Reductase
Glutathione
4-hydroxybenzaldehyde