Copy-number variation of housekeeping gene rpl13a in rat strains selected for nervous system excitability

Ruslan Kalendar; Alexander Belyayev; Tatiana Zachepilo; Alexander Vaido; Dmitry Maidanyuk; Alan H Schulman; Natalia Dyuzhikova

doi:10.1016/j.mcp.2017.02.004

Copy-number variation of housekeeping gene rpl13a in rat strains selected for nervous system excitability

Mol Cell Probes. 2017 Jun:33:11-15. doi: 10.1016/j.mcp.2017.02.004. Epub 2017 Feb 10.

Authors

Ruslan Kalendar¹, Alexander Belyayev², Tatiana Zachepilo³, Alexander Vaido⁴, Dmitry Maidanyuk⁵, Alan H Schulman⁶, Natalia Dyuzhikova⁷

Affiliations

¹ LUKE/BI Plant Genome Dynamics, Institute of Biotechnology and Viikki Plant Science Centre, University of Helsinki, P.O. Box 65, FI-00014 Helsinki, Finland. Electronic address: ruslan.kalendar@mail.ru.
² Laboratory of Molecular Cytogenetics and Karyology, Institute of Botany, Czech Academy of Sciences, Zámek 1, CZ-25243 Průhonice, Czech Republic. Electronic address: alexander.belyayev@ibot.cas.cz.
³ Laboratory of Genetics of Higher Nervous Activity, Pavlov Institute of Physiology of the Russian Academy of Science, Nab. Makarova, 6, 199034 Saint Petersburg, Russia. Electronic address: polosataya2@mail.ru.
⁴ Laboratory of Genetics of Higher Nervous Activity, Pavlov Institute of Physiology of the Russian Academy of Science, Nab. Makarova, 6, 199034 Saint Petersburg, Russia. Electronic address: aivaido@mail.ru.
⁵ Laboratory of Genomic and Proteomic Research, Immanuel Kant Baltic Federal University, 236041 Kaliningrad, Russia. Electronic address: DMaidaniuk@kantiana.ru.
⁶ LUKE/BI Plant Genome Dynamics, Institute of Biotechnology and Viikki Plant Science Centre, University of Helsinki, P.O. Box 65, FI-00014 Helsinki, Finland; Green Technology, Natural Resources Institute Finland (Luke), Latokartanonkaari 9, FI-00790 Helsinki, Finland. Electronic address: alan.schulman@helsinki.fi.
⁷ Laboratory of Genetics of Higher Nervous Activity, Pavlov Institute of Physiology of the Russian Academy of Science, Nab. Makarova, 6, 199034 Saint Petersburg, Russia. Electronic address: dyuzhikova@mail.ru.

PMID: 28192165
DOI: 10.1016/j.mcp.2017.02.004

Abstract

We evaluated copy number variation (CNV) for four genes in rat strains differing in nervous system excitability. rpl13a copy number is significantly reduced in hippocampus and bone marrow in rats with a high excitability threshold and stress. The observed phenomenon may be associated with a role for rpl13a in lipid metabolism.

Keywords: Bone marrow; Copy number variation (CNV); Hippocampus; Nervous system excitability; Quantitative real-time multicolor multiplex PCR (qmPCR); Rat.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Marrow / metabolism
Bone Marrow / physiology
Cortical Excitability / genetics
Cortical Excitability / physiology
DNA Copy Number Variations / genetics*
Hippocampus / metabolism
Hippocampus / physiology
Nervous System Physiological Phenomena / genetics*
Rats
Ribosomal Proteins / genetics*

Substances

Ribosomal Proteins
Rpl13a protein, rat