Rapid detection of Enterovirus and Coxsackievirus A10 by a TaqMan based duplex one-step real time RT-PCR assay

Mol Cell Probes. 2017 Jun:33:8-10. doi: 10.1016/j.mcp.2017.02.003. Epub 2017 Feb 8.

Abstract

A TaqMan based duplex one-step real time RT-PCR (rRT-PCR) assay was developed for the rapid detection of Coxsackievirus A10 (CV-A10) and other enterovirus (EVs) in clinical samples. The assay was fully evaluated and found to be specific and sensitive. When applied in 115 clinical samples, a 100% diagnostic sensitivity in CV-A10 detection and 97.4% diagnostic sensitivity in other EVs were found.

Keywords: Coxsackievirus A10; Enterovirus; Hand-foot-mouth disease; Real time RT-PCR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Viral / genetics
  • Antigens, Viral / isolation & purification
  • Enterovirus / genetics*
  • Enterovirus / isolation & purification*
  • Enterovirus / pathogenicity
  • Enterovirus Infections / diagnosis*
  • Enterovirus Infections / genetics
  • Enterovirus Infections / virology
  • Genotype
  • Humans
  • Molecular Diagnostic Techniques*

Substances

  • Antigens, Viral