Knockdown of linc-OIP5 inhibits proliferation and migration of glioma cells through down-regulation of YAP-NOTCH signaling pathway

Gene. 2017 Apr 30:610:24-31. doi: 10.1016/j.gene.2017.02.006. Epub 2017 Feb 9.

Abstract

Long intergenic noncoding RNAs (lincRNAs) play important roles in regulating the biological functions and underlying molecular mechanisms of glioma. Here, we investigated the expression level and biological function of linc-OIP5 in glioma. In the current study, we used quantitative real-time polymerase chain reaction (qRT-PCR) to determine the expression of linc-OIP5 in glioma tissues and in adjacent normal tissues. Level of linc-OIP5 was up-regulated in glioma tissues and significantly correlated with the advanced tumor stage (III/IV). Subsequently, the efficacy of knockdown of linc-OIP5 by linc-OIP5-small interfering RNA (siRNA) was evaluated in vitro, and we found that knockdown of linc-OIP5 can inhibit glioma cells proliferation, migration in vitro and tumor formation in vivo. Further mechanistic studies revealed the effect of linc-OIP5 knockdown on glioma cell phenotype at least partially through down-regulation of YAP and inhibition of Notch signaling pathway activity. Thus, our study provides evidence that linc-OIP5 is a potential therapeutic target and novel molecular biomarker for glioma.

Keywords: Glioma; Linc-OIP5; Migration; Proliferation; Signaling pathway.

MeSH terms

  • Animals
  • Cell Cycle Proteins
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Central Nervous System Neoplasms / genetics*
  • Central Nervous System Neoplasms / metabolism
  • Chromosomal Proteins, Non-Histone / genetics
  • Down-Regulation
  • Female
  • Gene Knockdown Techniques
  • Glioma / genetics*
  • Glioma / metabolism
  • Humans
  • Male
  • Mice
  • Mice, Nude
  • Middle Aged
  • RNA, Long Noncoding / genetics
  • RNA, Long Noncoding / metabolism*
  • Retrospective Studies
  • Signal Transduction*

Substances

  • Cell Cycle Proteins
  • Chromosomal Proteins, Non-Histone
  • OIP5 protein, human
  • RNA, Long Noncoding
  • long noncoding RNA OIP5, human