Surfactant protein A (SP-A) and SP-A-derived peptide attenuate chemotaxis of mast cells induced by human β-defensin 3

Yasuaki Uehara; Motoko Takahashi; Masaki Murata; Atsushi Saito; Rina Takamiya; Yoshihiro Hasegawa; Koji Kuronuma; Hirofumi Chiba; Jiro Hashimoto; Norimasa Sawada; Hiroki Takahashi; Yoshio Kuroki; Shigeru Ariki

doi:10.1016/j.bbrc.2017.02.028

Surfactant protein A (SP-A) and SP-A-derived peptide attenuate chemotaxis of mast cells induced by human β-defensin 3

Biochem Biophys Res Commun. 2017 Mar 25;485(1):107-112. doi: 10.1016/j.bbrc.2017.02.028. Epub 2017 Feb 8.

Authors

Affiliations

¹ Department of Biochemistry, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan; Department of Respiratory Medicine and Allergology, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan.
² Department of Biochemistry, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan.
³ Department of Pathology, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan.
⁴ Department of Respiratory Medicine and Allergology, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan.
⁵ Department of Biochemistry, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan; Department of Urologic Surgery and Andrology, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan.
⁶ Department of Biochemistry, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Japan; Department of Chemistry, Sapporo Medical University Center for Medical Education, S-1 W-17, Chuo-ku, Sapporo, Japan. Electronic address: sarikscb@sapmed.ac.jp.

PMID: 28188794
DOI: 10.1016/j.bbrc.2017.02.028

Abstract

Human β-defensin 3 (hBD3) is known to be involved in mast cell activation. However, molecular mechanisms underlying the regulation of hBD3-induced mast cell activation have been poorly understood. We previously reported that SP-A and SP-A-derived peptide 01 (SAP01) regulate the function of hBD3. In this study, we focused on the effects of SP-A and SAP01 on the activation of mast cells induced by hBD3. SAP01 directly bound to hBD3. Mast cell-mediated vascular permeability and edema in hBD3 administered rat ears were decreased when injected with SP-A or SAP01. Compatible with the results in rat ear model, both SP-A and SAP01 inhibited hBD3-induced chemotaxis of mast cells in vitro. Direct interaction between SP-A or SAP01 and hBD3 seemed to be responsible for the inhibitory effects on chemotaxis. Furthermore, SAP01 attenuated hBD3-induced accumulation of mast cells and eosinophils in tracheas of the OVA-sensitized inflammatory model. SP-A might contribute to the regulation of inflammatory responses mediated by mast cells during infection.

Keywords: Chemotaxis; Human β-defensin 3; Mast cells; Surfactant protein A.

MeSH terms

Animals
Capillary Permeability / drug effects
Chemotaxis / drug effects*
Edema / drug therapy
Edema / immunology
Humans
Inflammation / drug therapy
Inflammation / immunology*
Male
Mast Cells / cytology
Mast Cells / drug effects
Mast Cells / immunology*
Peptides / chemistry
Peptides / pharmacology
Pulmonary Surfactant-Associated Protein A / chemistry
Pulmonary Surfactant-Associated Protein A / immunology*
Pulmonary Surfactant-Associated Protein A / pharmacology
Rats, Sprague-Dawley
beta-Defensins / immunology*

Substances

DEFB103A protein, human
Peptides
Pulmonary Surfactant-Associated Protein A
beta-Defensins