Rapid diagnosis of Vibrio owensii responsible for shrimp acute hepatopancreatic necrosis disease with isothermal recombinase polymerase amplification assay

Liyuan Liu; Luzhi Jiang; Yongxin Yu; Xiaoming Xia; Yingjie Pan; Shuling Yan; Yongjie Wang

doi:10.1016/j.mcp.2017.02.001

Rapid diagnosis of Vibrio owensii responsible for shrimp acute hepatopancreatic necrosis disease with isothermal recombinase polymerase amplification assay

Mol Cell Probes. 2017 Jun:33:4-7. doi: 10.1016/j.mcp.2017.02.001. Epub 2017 Feb 5.

Authors

Liyuan Liu¹, Luzhi Jiang¹, Yongxin Yu¹, Xiaoming Xia¹, Yingjie Pan², Shuling Yan³, Yongjie Wang⁴

Affiliations

¹ College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
² College of Food Science and Technology, Shanghai Ocean University, Shanghai, China; Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, China.
³ College of Food Science and Technology, Shanghai Ocean University, Shanghai, China; Institute of Biochemistry and Molecular Cell Biology, University of Göttingen, Göttingen, Germany.
⁴ College of Food Science and Technology, Shanghai Ocean University, Shanghai, China; Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, China. Electronic address: yjwang@shou.edu.cn.

PMID: 28179103
DOI: 10.1016/j.mcp.2017.02.001

Abstract

A rapid and sensitive AHPND-RPA assay was developed for the specific detection of the AHPND-causing Vibrio owensii. The AHPND-RPA detected as few as 2 copies per reaction in 9.02 ± 0.66 min at 39 °C, and showed 100% positive predictive value and negative predictive value for AHPND diagnosis.

Keywords: Acute hepatopancreatic necrosis disease; Diagnosis; RPA; Recombinase polymerase amplification; Vibrio owensii.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animal Diseases / diagnosis*
Animal Diseases / genetics
Animal Diseases / microbiology
Animals
Hepatopancreas / microbiology
Hepatopancreas / pathology
Host-Pathogen Interactions / genetics
Seafood / microbiology*
Vibrio / genetics*
Vibrio / isolation & purification*
Vibrio / pathogenicity