Opuntia ficus-indica cladodes as a functional ingredient: bioactive compounds profile and their effect on antioxidant quality of bread

Lotfi Msaddak; Ola Abdelhedi; Amani Kridene; Mostafa Rateb; Lassaâd Belbahri; Emna Ammar; Moncef Nasri; Nacim Zouari

doi:10.1186/s12944-016-0397-y

Opuntia ficus-indica cladodes as a functional ingredient: bioactive compounds profile and their effect on antioxidant quality of bread

Lipids Health Dis. 2017 Feb 7;16(1):32. doi: 10.1186/s12944-016-0397-y.

Authors

Lotfi Msaddak¹, Ola Abdelhedi¹, Amani Kridene², Mostafa Rateb³, Lassaâd Belbahri⁴, Emna Ammar², Moncef Nasri¹, Nacim Zouari⁵

Affiliations

¹ Laboratory of Enzyme Engineering and Microbiology, Engineering National School of Sfax (ENIS), University of Sfax, Sfax, Tunisia.
² Research Unit of Coastal and Urban Environments, ENIS, University of Sfax, Sfax, Tunisia.
³ School of Science and Sport, University of West of Scotland, Paisley, UK.
⁴ Laboratory of Soil Biology, University of Neuchatel, Neuchatel, Switzerland.
⁵ High Institute of Applied Biology of Medenine (ISBAM), University of Gabes, Medenine, Tunisia. znacim2002@yahoo.fr.

Abstract

Background: In the context of a balanced diet, the antioxidant-rich food consumption is a preventive way of many degenerative diseases. Consequently, improving the nutraceutical quality of traditional foods such as bakery products is an interesting approach. Considering the present consumer's demand, cladodes from prickly pear that were traditionally used as a valuable food as well as in folk medicine for the treatment of several chronic diseases were investigated for their use in bread production to improve its functionality.

Methods: Bioactive substances were determined by liquid chromatography-high resolution electrospray ionization mass spectrometry (LC-HRESIMS) analysis. Dough rheological properties were characterized by alveographic measurements. Bread antioxidant quality was evaluated by total phenolics content, DPPH• radical-scavenging, metal (Fe²⁺) chelating and Fe³⁺ reducing power determinations.

Results: LC-HRESIMS analysis of the cladodes extract allowed the identification of 9 flavonoids, 2 phenolics, 1 alkaloid and 1 terpenoid compounds. Cladodes powder enrichment induced important modifications on the dough rheological parameters in terms of the extensibility (L) and deformation energy (W) decrease. Moreover, cladodes powder addition to bread resulted in a decrease in both crust and crumb colour parameters (L*, a* and b*). A 5% supplementation resulted in an increase of the bread yield and bread specific volume by 8.9 and 25%, respectively. Interestingly, Bread containing cladodes powder showed enhanced total phenolics content and antioxidant potential as compared to the control.

Conclusions: Substitution of wheat flour by the cladodes powder at 5% level was optimal for improving the total phenolics content and the antioxidant potential of bread without having any negative effect on its sensory acceptability. Cladodes from Opuntia ficus-indica could be considered as a potential health-promoting functional ingredient in bakery products.

Keywords: Antioxidant; Bread quality; Cladodes; Dough; Flavonoids; LC-HRESIMS; Opuntia ficus-indica.

MeSH terms

Antioxidants / analysis*
Bread / analysis*
Flavonoids / analysis*
Opuntia / chemistry*
Plant Preparations / analysis

Substances

Antioxidants
Flavonoids
Plant Preparations