Pinicolol B from Antrodia cinnamomea induces apoptosis of nasopharyngeal carcinoma cells

Tsung-Ru Wu; Tsung-Teng Huang; Jan Martel; Jian-Ching Liau; Chen-Yaw Chiu; Yann-Lii Leu; Wei-Ting Jian; I-Te Chang; Chia-Chen Lu; David M Ojcius; Yun-Fei Ko; Hsin-Chih Lai; John D Young

doi:10.1016/j.jep.2017.02.008

Pinicolol B from Antrodia cinnamomea induces apoptosis of nasopharyngeal carcinoma cells

J Ethnopharmacol. 2017 Apr 6:201:117-122. doi: 10.1016/j.jep.2017.02.008. Epub 2017 Feb 4.

Authors

Tsung-Ru Wu¹, Tsung-Teng Huang², Jan Martel³, Jian-Ching Liau⁴, Chen-Yaw Chiu⁵, Yann-Lii Leu⁶, Wei-Ting Jian⁴, I-Te Chang⁴, Chia-Chen Lu⁷, David M Ojcius⁸, Yun-Fei Ko⁹, Hsin-Chih Lai¹⁰, John D Young¹¹

Affiliations

¹ Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan; Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan.
² Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan; Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan; Center for Molecular and Clinical Immunology, Chang Gung University, Taoyuan 33302, Taiwan; Chang Gung Immunology Consortium, Linkou Chang Gung Memorial Hospital, Chang Gung University, Taoyuan 33302, Taiwan; Research Center of Bacterial Pathogenesis, Chang Gung University, Taoyuan 33302, Taiwan.
³ Center for Molecular and Clinical Immunology, Chang Gung University, Taoyuan 33302, Taiwan; Chang Gung Immunology Consortium, Linkou Chang Gung Memorial Hospital, Chang Gung University, Taoyuan 33302, Taiwan; Laboratory of Nanomaterials, Chang Gung University, Taoyuan 33302, Taiwan.
⁴ Chang Gung Biotechnology Corporation, Taipei 10508, Taiwan.
⁵ Biochemical Engineering Research Center, Ming Chi University of Technology, New Taipei City 24301, Taiwan.
⁶ Graduate Institute of Natural Products, Chang Gung University, Taoyuan 33302, Taiwan.
⁷ Department of Respiratory Therapy, Fu Jen Catholic University, New Taipei City 24205, Taiwan.
⁸ Center for Molecular and Clinical Immunology, Chang Gung University, Taoyuan 33302, Taiwan; Chang Gung Immunology Consortium, Linkou Chang Gung Memorial Hospital, Chang Gung University, Taoyuan 33302, Taiwan; Department of Biomedical Sciences, University of the Pacific, Arthur Dugoni School of Dentistry, San Francisco, CA 94103, United States.
⁹ Chang Gung Immunology Consortium, Linkou Chang Gung Memorial Hospital, Chang Gung University, Taoyuan 33302, Taiwan; Chang Gung Biotechnology Corporation, Taipei 10508, Taiwan; Biochemical Engineering Research Center, Ming Chi University of Technology, New Taipei City 24301, Taiwan.
¹⁰ Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan; Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan; Center for Molecular and Clinical Immunology, Chang Gung University, Taoyuan 33302, Taiwan; Chang Gung Immunology Consortium, Linkou Chang Gung Memorial Hospital, Chang Gung University, Taoyuan 33302, Taiwan; Research Center of Bacterial Pathogenesis, Chang Gung University, Taoyuan 33302, Taiwan; Research Center for Industry of Human Ecology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 33303, Taiwan; Graduate Institute of Health Industry and Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 33303, Taiwan. Electronic address: hclai@mail.cgu.edu.tw.
¹¹ Center for Molecular and Clinical Immunology, Chang Gung University, Taoyuan 33302, Taiwan; Chang Gung Immunology Consortium, Linkou Chang Gung Memorial Hospital, Chang Gung University, Taoyuan 33302, Taiwan; Laboratory of Nanomaterials, Chang Gung University, Taoyuan 33302, Taiwan; Chang Gung Biotechnology Corporation, Taipei 10508, Taiwan; Biochemical Engineering Research Center, Ming Chi University of Technology, New Taipei City 24301, Taiwan; Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, NY 10021, United States. Electronic address: jdyoung@mail.cgu.edu.tw.

PMID: 28167294
DOI: 10.1016/j.jep.2017.02.008

Abstract

Ethnopharmacological relevance: The medicinal mushroom Antrodia cinnamomea possesses anticancer properties but the active compounds responsible for these effects are mostly unknown.

Aim of the study: We aimed to identify novel A. cinnamomea compounds that produce cytotoxic effects on cancer cells.

Materials and methods: Using ethanol extraction and chromatography, we isolated the lanostanoid compound lanosta-7,9(11),24-trien-3β,15α,21-triol (1) from cultured A. cinnamomea mycelium. Cytotoxicity and pro-apoptotic effects of compound 1 were evaluated using the MTS assay and flow cytometry analysis, respectively.

Results: Compound 1 produced cytotoxic effects on the nasopharyngeal carcinoma cell lines TW02 and TW04, with IC₅₀ values of 63.3 and 115.0μM, respectively. On the other hand, no cytotoxic effects were observed on non-tumorigenic nasopharyngeal epithelial cells (NP69). In addition, compound 1 induced apoptosis in TW02 and TW04 cells as revealed by flow cytometry analysis.

Conclusions: Our results demonstrate for the first time the presence of pinicolol B in A. cinnamomea mycelium and suggest that this compound may contribute to the anticancer effects of A. cinnamomea.

Keywords: Antrodia cinnamomea; Cell death; Cytotoxicity; Medicinal mushroom; Nasopharyngeal carcinoma.

MeSH terms

Antineoplastic Agents / pharmacology*
Antrodia*
Apoptosis / drug effects
Carcinoma / drug therapy
Cell Line
Cell Line, Tumor
Cell Survival / drug effects
Humans
Mycelium
Nasopharyngeal Carcinoma
Nasopharyngeal Neoplasms / drug therapy
Triterpenes / pharmacology*

Substances

Antineoplastic Agents
Triterpenes
pinicolol B