Actinomadura Species: Laboratory Maintenance and Ribosome Engineering

Dipesh Dhakal; Nguyen Thanh Chung; Vijay Rayamajhi; Jae Kyung Sohng

doi:10.1002/cpmc.22

Actinomadura Species: Laboratory Maintenance and Ribosome Engineering

Curr Protoc Microbiol. 2017 Feb 6:44:10G.1.1-10G.1.12. doi: 10.1002/cpmc.22.

Authors

Dipesh Dhakal¹, Nguyen Thanh Chung¹, Vijay Rayamajhi¹, Jae Kyung Sohng^{1

2}

Affiliations

¹ Department of Life Science and Biochemical Engineering, Sun Moon University, Asan-si, Republic of Korea.
² Department of BT-Convergent Pharmaceutical Engineering, Sun Moon University, Asan-si, Republic of Korea.

PMID: 28166386
DOI: 10.1002/cpmc.22

Abstract

Actinomadura spp. are aerobic, Gram-positive, catalase-positive, non-acid fast, non-motile actinomycetes. Some species of Actinomadura are associated with opportunistic infections in humans. However, many bioactive compounds with pharmaceutical applications can be isolated from various Actinomadura spp. This unit includes general protocols for the laboratory maintenance of Actinomadura spp., including growth in liquid medium, growth on solid agar, long-term storage, and generation of a higher producing strain by ribosome engineering. Actinomadura hibisca P157-2 is used as a prototype for explaining the considerations for efficient laboratory maintenance of Actinomadura spp. © 2017 by John Wiley & Sons, Inc.

Keywords: Actinomadura spp; growth medium; ribosome engineering; storage.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actinobacteria / genetics*
Actinobacteria / growth & development*
Bacteriological Techniques / methods*
Culture Media / chemistry
Genetics, Microbial / methods*
Metabolic Engineering / methods*
Molecular Biology / methods*
Preservation, Biological / methods
Ribosomes / genetics*

Substances

Culture Media