Abstract
Four biflavonoid glycosides, balsamisides A-D (1-4), and nine known compounds (5-13) were obtained from the white petals of Impatiens balsamina. The 2D structures of the purified phytochemicals were established using conventional NMR techniques in addition to the new long-range HSQMBC NMR experiment. Acid hydrolysis followed by experimental and quantum-mechanics-based ECD data analysis permitted full configurational assignment of the purified metabolites. Compounds 1-13 were assessed for their potential to impede the generation of nitric oxide in lipopolysaccharide-stimulated BV2 cells. They were also investigated for potential neuroprotective activity using C6 cells and cytotoxicity against some human tumor cell lines, but were inactive (IC50 > 10 μM) against all the cell lines.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Algorithms
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / chemistry
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Anti-Inflammatory Agents, Non-Steroidal / isolation & purification*
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Biflavonoids / chemistry
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Biflavonoids / isolation & purification*
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Biflavonoids / pharmacology*
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Glycosides / chemistry
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Glycosides / isolation & purification*
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Glycosides / pharmacology*
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Humans
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Impatiens / chemistry*
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects
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Mice
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Molecular Structure
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Neuroprotective Agents / chemistry
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Neuroprotective Agents / isolation & purification*
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Neuroprotective Agents / pharmacology*
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Nitric Oxide / biosynthesis
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Nuclear Magnetic Resonance, Biomolecular
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Republic of Korea
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Biflavonoids
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Glycosides
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Lipopolysaccharides
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Neuroprotective Agents
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Nitric Oxide