[Effects of complex of 3,6-di-(dimethylamino)-dibenzopyriodonium with praseodymium dicitrate on the syntheses of DNA, RNA, protein, nucleoprotein and ATP of leukemia L 7712 cells in mice]

Zhongguo Yao Li Xue Bao. 1989 Mar;10(2):188-91.
[Article in Chinese]

Abstract

The incorporations of [3H] thymidine, [3H]uridine and [3H]leucine into DNA, RNA and protein synthesis in leukemia 7712 cells were inhibited by the complex of 3,6-di-(dimethylamino)-dibenzopyriodonium with praseodymium (Pr, rare earth element) dicitrite 34 micrograms/ml for 3-24 h. The degree of inhibition increased in proportion to the incubation time. After being treated with [C17H20N2I]3[Pr(C6H5O7)2] 34 micrograms/ml for 3, 6, 12 and 24 h, the incorporation of [32P]Na2HPO4 into the nucleoprotein of leukemia 7712 cells was inhibited by 49, 57, 65 and 85%, while those into ATP were inhibited by 43, 59, 65 and 83%, respectively. The ID50 of [C17H20N2I]3[Pr(C6H5O7)2] on DNA synthesis in leukemia 7712 cells at 24 h was 22 micrograms/ml. After the complex was removed from the medium entirely, the rate of DNA synthesis decreased with time over 3-12 h. This result indicated that the inhibition mechanism was likely due to damage to the DNA template.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / biosynthesis*
  • Animals
  • Benzopyrenes / pharmacology*
  • DNA, Neoplasm / biosynthesis*
  • DNA, Neoplasm / drug effects
  • Leukemia, Experimental / metabolism*
  • Mice
  • Neoplasm Proteins / biosynthesis
  • Nucleoproteins / biosynthesis
  • Onium Compounds / pharmacology*
  • Organometallic Compounds / pharmacology*
  • RNA, Neoplasm / biosynthesis*
  • RNA, Neoplasm / drug effects

Substances

  • Benzopyrenes
  • DNA, Neoplasm
  • Neoplasm Proteins
  • Nucleoproteins
  • Onium Compounds
  • Organometallic Compounds
  • RNA, Neoplasm
  • 3,6-di(dimethylamino)dibenzopyriodonium praseodymium dicitrate complex
  • Adenosine Triphosphate