Histochemical Analysis of Plant Secretory Structures

Methods Mol Biol. 2017:1560:313-330. doi: 10.1007/978-1-4939-6788-9_24.

Abstract

Histochemical analysis is essential for the study of plant secretory structures whose classification is based, at least partially, on the composition of their secretion. As each gland may produce one or more types of substances, a correct analysis of its secretion should be done using various histochemical tests to detect metabolites of different chemical classes. Here I describe some of the most used methods to detect carbohydrates, proteins, lipids, phenolic compounds, and alkaloids in the secretory structures.

Keywords: Classification; Function; Histochemistry; Plant secretory structures; Secondary metabolites; Secretion.

MeSH terms

  • Carbohydrate Metabolism
  • Carbohydrates / chemistry
  • Histocytochemistry / methods*
  • Plant Cells / metabolism*
  • Plant Mucilage / chemistry
  • Plant Mucilage / metabolism
  • Plant Proteins / chemistry
  • Plant Proteins / metabolism
  • Plant Structures / cytology*
  • Plants / metabolism*
  • Secondary Metabolism
  • Starch / chemistry
  • Starch / metabolism

Substances

  • Carbohydrates
  • Plant Mucilage
  • Plant Proteins
  • Starch