Staining of Lipid Droplets with Monodansylpentane

Methods Mol Biol. 2017:1560:231-236. doi: 10.1007/978-1-4939-6788-9_17.

Abstract

Monodansylpentane (MDH) is a fluorophore that displays selective labeling of lipid droplets (LDs). The dye preferentially segregates into the neutral lipid cores of LDs and emits blue fluorescence, compatible with the simultaneous use of green and red fluorescent reporters in multi-color live-cell imaging. MDH can be used for visualizing LDs not only in cell cultures, but also in fixed tissues such as the fat body and ovaries from Drosophila. MDH is therefore a versatile marker for LDs in fluorescence microscopy.

Keywords: Drosophila; Fluorescence microscopy; Lipid droplets; Live-cell imaging; Monodansylpentane; Tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Dansyl Compounds*
  • Drosophila
  • Fluorescent Dyes*
  • Humans
  • Lipid Droplets*
  • Microscopy, Fluorescence* / methods
  • Staining and Labeling* / methods

Substances

  • Dansyl Compounds
  • Fluorescent Dyes
  • monodansylpentane