The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium

Methods Mol Biol. 2017:1560:133-144. doi: 10.1007/978-1-4939-6788-9_9.

Abstract

Lectin histochemistry is commonly used to characterize the pattern of glycoconjugates in cells and tissues. Recent studies show that alterations in these glycoconjugates are associated with the entry of cells into apoptosis. A widely used technique for the detection of apoptotic cell death is TUNEL. In this chapter, we study the sensitivity of both techniques to identify apoptotic cells in the testis of photo-inhibited Syrian hamster.

Keywords: Apoptosis; Germ cell; Lectin histochemistry; Sensitivity; Syrian hamster; TUNEL; Testis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis*
  • Biomarkers
  • Cricetinae
  • Germ Cells / metabolism
  • Glycoconjugates
  • Histocytochemistry / methods*
  • In Situ Nick-End Labeling / methods
  • Lectins / metabolism*
  • Male
  • Seminiferous Epithelium / metabolism*
  • Sensitivity and Specificity
  • Testis / cytology
  • Testis / metabolism

Substances

  • Biomarkers
  • Glycoconjugates
  • Lectins