Non-target analysis of phospholipid and sphingolipid species in egg yolk using liquid chromatography/triple quadrupole tandem mass spectrometry

Bogusław Buszewski; Justyna Walczak; Petar Žuvela; J Jay Liu

doi:10.1016/j.chroma.2017.01.055

Non-target analysis of phospholipid and sphingolipid species in egg yolk using liquid chromatography/triple quadrupole tandem mass spectrometry

J Chromatogr A. 2017 Mar 3:1487:179-186. doi: 10.1016/j.chroma.2017.01.055. Epub 2017 Jan 24.

Authors

Bogusław Buszewski¹, Justyna Walczak², Petar Žuvela³, J Jay Liu³

Affiliations

¹ Department of Environmental Chemistry and Bioanalytics Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Torun, Poland; Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Wileńska 4, 87-100 Toruń, Poland. Electronic address: bbusz@chem.umk.pl.
² Department of Environmental Chemistry and Bioanalytics Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Torun, Poland.
³ Department of Chemical Engineering, Pukyong National University, 365 Sinseon-ro, 48-547, Busan, South Korea.

PMID: 28139226
DOI: 10.1016/j.chroma.2017.01.055

Abstract

In this work, phospholipids extracted from egg yolk (control group, experimental group) were identified using high performance liquid chromatography coupled with electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS). Combinations of fatty acyls occurring in 11 classes of phospholipids from egg yolk were investigated. Differences between the profile of fatty acyls from hens fed traditionally and the ones that received special diet supplementation were observed. Experimental findings were complemented with multivariate chemometric analysis. Multiple reaction monitoring mass spectrometry mode was utilized and 123 distinct combinations of fatty acyls occurring in phospholipids were identified. From these, large portions are polyunsaturated fatty acyls from the omega-3 and omega-6 family. HPLC MS/MS analysis allows for quick, accurate and precise determination of biologically active compounds, found in low concentrations within the tested material.

Keywords: Egg yolk; Fatty acyls; HPLC–MS/MS; MRM; Phospholipids.

MeSH terms

Animals
Chickens
Chromatography, High Pressure Liquid*
Egg Yolk / chemistry*
Fatty Acids, Omega-3 / analysis
Fatty Acids, Omega-6 / analysis
Female
Phospholipids / analysis*
Phospholipids / chemistry
Spectrometry, Mass, Electrospray Ionization
Sphingolipids / analysis*
Tandem Mass Spectrometry*

Substances

Fatty Acids, Omega-3
Fatty Acids, Omega-6
Phospholipids
Sphingolipids