An innovative ring-shaped electroeluter for high concentration preparative isolation of protein from polyacrylamide gel

Jing-Jing Chen; Guo-Qing Li; Amit Pratush; Sharmin Jahan; Fan-Zhi Kong; Hua Xiao; Liu-Yin Fan; Cheng-Xi Cao

doi:10.1016/j.ab.2017.01.023

An innovative ring-shaped electroeluter for high concentration preparative isolation of protein from polyacrylamide gel

Anal Biochem. 2017 Apr 15:523:39-43. doi: 10.1016/j.ab.2017.01.023. Epub 2017 Jan 27.

Authors

Jing-Jing Chen¹, Guo-Qing Li¹, Amit Pratush¹, Sharmin Jahan¹, Fan-Zhi Kong¹, Hua Xiao¹, Liu-Yin Fan², Cheng-Xi Cao³

Affiliations

¹ Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
² Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: lyfan@sjtu.edu.cn.
³ Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: cxcao@sjtu.edu.cn.

PMID: 28137604
DOI: 10.1016/j.ab.2017.01.023

Abstract

A ring-shaped electroeluter (RSE) was designed for protein recovery from polyacrylamide gel matrix. The RSE was designed in such a way that a ring-shaped well was used to place gel slices and an enrichment well was used to collect eluted protein samples. With HSA as model protein, the electroelution time was less than 30 min with 80% recovery rate, and the concentration of recovered protein was 50 times higher than that of conventional method. The RSE could be reused at least ten times. The developed device makes great advance towards economic electroelution of biomolecules (such as proteins) from gel matrix.

Keywords: Electroelution; Gel electrophoresis; Preparative isolation; Protein; Sample recovery.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acrylic Resins / chemistry*
Electrochemistry / instrumentation*
Electrophoresis, Polyacrylamide Gel / methods*
Humans
Serum Albumin / isolation & purification*

Substances

Acrylic Resins
Serum Albumin
polyacrylamide gels