Spatial Metabolite Profiling by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging

Berin A Boughton; Brett Hamilton

doi:10.1007/978-3-319-47656-8_12

Spatial Metabolite Profiling by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging

Adv Exp Med Biol. 2017:965:291-321. doi: 10.1007/978-3-319-47656-8_12.

Authors

Berin A Boughton¹, Brett Hamilton^{2

3}

Affiliations

¹ Metabolomics Australia, School of BioSciences, The University of Melbourne, Melbourne, VIC, 3010, Australia. baboug@unimelb.edu.au.
² Mater Research Institute, The University of Queensland, St Lucia, QLD, Australia.
³ Pathology Department, Mater Health Services, South Brisbane, QLD, Australia.

PMID: 28132185
DOI: 10.1007/978-3-319-47656-8_12

Abstract

Mass spectrometry imaging (MSI) is rapidly maturing as an advanced method for spatial metabolite profiling. Herein, we provide an introduction to MSI including types of instrumentation, detailed sample preparation, data collection, overview of data analysis steps, software, common standards, and new developments. Further, we provide an overview of MSI in the clinical space over the past 3 years where MSI has been deployed in diverse research areas including cancer, neurobiology, lipidomics, and metabolite profiling and mapping to name only a few. We provide several examples demonstrating the applicability of MSI to spatially profile metabolites in unique systems requiring special considerations outside of the norm.

Keywords: High resolution; MALDI; Mass spectrometry imaging; Matrix; Spatial metabolomics.

Publication types

Review

MeSH terms

Data Interpretation, Statistical
Humans
Lipids / analysis
Metabolomics / methods*
Proteins / analysis
Specimen Handling
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Lipids
Proteins