M13-Tailed Simple Sequence Repeat (SSR) Markers in Studies of Genetic Diversity and Population Structure of Common Oat Germplasm

Agnieszka Onyśk; Maja Boczkowska

doi:10.1007/978-1-4939-6682-0_12

M13-Tailed Simple Sequence Repeat (SSR) Markers in Studies of Genetic Diversity and Population Structure of Common Oat Germplasm

Methods Mol Biol. 2017:1536:159-168. doi: 10.1007/978-1-4939-6682-0_12.

Authors

Agnieszka Onyśk¹, Maja Boczkowska²

Affiliations

¹ Department of Functional Genomics, Plant Breeding and Acclimatization Institute - National Research Institute, Błonie, Poland.
² National Centre for Plant Genetic Resources, Plant Breeding and Acclimatization Institute - National Research Institute, Radzików, 05-870, Błonie, Poland. m.boczkowska@ihar.edu.pl.

PMID: 28132150
DOI: 10.1007/978-1-4939-6682-0_12

Abstract

Simple Sequence Repeat (SSR) markers are one of the most frequently used molecular markers in studies of crop diversity and population structure. This is due to their uniform distribution in the genome, the high polymorphism, reproducibility, and codominant character. Additional advantages are the possibility of automatic analysis and simple interpretation of the results. The M13 tagged PCR reaction significantly reduces the costs of analysis by the automatic genetic analyzers. Here, we also disclose a short protocol of SSR data analysis.

Keywords: Avena sativa L; Diversity; Germplasm; M13; Population structure; SSR.

MeSH terms

Alleles
Avena / genetics*
Bacteriophage M13 / genetics*
Databases, Nucleic Acid
Genetic Markers*
Genetic Variation*
Microsatellite Repeats*
Polymerase Chain Reaction
Seeds / genetics*

Substances

Genetic Markers