Oat Doubled Haploids Following Maize Pollination

Philip A Davies; Parminder K Sidhu

doi:10.1007/978-1-4939-6682-0_2

Oat Doubled Haploids Following Maize Pollination

Methods Mol Biol. 2017:1536:23-30. doi: 10.1007/978-1-4939-6682-0_2.

Authors

Philip A Davies¹, Parminder K Sidhu²

Affiliations

¹ South Australian Research and Development Institute, GPO Box 397, Adelaide, SA, 5001, Australia. Phil.Davies@sa.gov.au.
² South Australian Research and Development Institute, GPO Box 397, Adelaide, SA, 5001, Australia.

PMID: 28132140
DOI: 10.1007/978-1-4939-6682-0_2

Abstract

Doubled haploids (DHs) are an important tool for the accelerated production of new crop varieties. In oat, DHs were first produced by pollinating oat florets with maize pollen. The resultant embryos spontaneously eliminate the maize chromosomes leaving a haploid complement of oat chromosomes. These embryos can be cultured in vitro using the "embryo rescue" technique to produce haploid plants whose chromosome number can be doubled with colchicine to produce homozygous DH oat plants.

Keywords: Avena sativa; Breeding; Doubled haploid; Haploid; Maize; Oat; Zea mays.

MeSH terms

Avena / genetics*
Avena / growth & development
Breeding
Crosses, Genetic
Haploidy*
Phenotype
Pollination*
Seeds / genetics
Zea mays / genetics*