Transcutaneous immunization of Streptococcus suis bacterin using dissolving microneedles

Kai-Jen Hsueh; Mei-Chin Chen; Li-Ting Cheng; Jai-Wei Lee; Wen-Bin Chung; Chun-Yen Chu

doi:10.1016/j.cimid.2016.12.001

Transcutaneous immunization of Streptococcus suis bacterin using dissolving microneedles

Comp Immunol Microbiol Infect Dis. 2017 Feb:50:78-87. doi: 10.1016/j.cimid.2016.12.001. Epub 2016 Dec 14.

Authors

Kai-Jen Hsueh¹, Mei-Chin Chen², Li-Ting Cheng³, Jai-Wei Lee⁴, Wen-Bin Chung⁵, Chun-Yen Chu⁶

Affiliations

¹ Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 91201 Taiwan; Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 91201 Taiwan.
² Department of Chemical Engineering, National Cheng Kung University, Tainan, 701 Taiwan.
³ Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 91201 Taiwan.
⁴ Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Pingtung, 91201 Taiwan.
⁵ Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 91201 Taiwan.
⁶ Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 91201 Taiwan. Electronic address: cychu@mail.npust.edu.tw.

PMID: 28131383
DOI: 10.1016/j.cimid.2016.12.001

Abstract

Vaccine delivery using microneedle (MN) patches is an easy, safe and painless alternative to traditional needle injections. In this study, we examined whether MN patches can enhance the efficacy of a Streptococcus suis serotype 2 (S. suis 2) vaccine in a mouse model. Results showed that MNs can reach 200-250μm into the skin, a depth beneficial for targeted delivery of antigens to antigen-presenting cells in the epidermis and dermis. Vaccination with prime-boost of MN induced higher levels of IgG2a antibody titer, T cell proliferation, and T_H1 cytokines (IFN-γ and IL-12) as compared to intramuscular (IM) injection. In addition, single dose MN vaccination better protected mice against lethal challenge than IM vaccination. MN vaccination also conferred long-term IgG2a antibody against S. suis 2 bacteria presence for up to 7 months. Taken together, these data showed that vaccine delivery by MNs results in superior immune response and protection rate when compared to IM injections.

Keywords: Microneedles (MNs); Streptococcus suis; Transcutaneous immunization.

Publication types

Evaluation Study

MeSH terms

Administration, Cutaneous
Animals
Cytokines / blood
Cytokines / immunology
Immunization Schedule
Immunoglobulin G / blood
Injections, Intramuscular
Mice
Mice, Inbred BALB C
Microinjections / methods
Needles
Skin / immunology*
Streptococcal Infections / immunology*
Streptococcal Infections / prevention & control
Streptococcal Vaccines / administration & dosage*
Streptococcus suis / immunology*
Th1 Cells / immunology
Transdermal Patch
Vaccination / methods*

Substances

Cytokines
Immunoglobulin G
Streptococcal Vaccines