Single Dose Caffeine Protects the Neonatal Mouse Brain against Hypoxia Ischemia

Max Winerdal; Vijay Urmaliya; Malin E Winerdal; Bertil B Fredholm; Ola Winqvist; Ulrika Ådén

doi:10.1371/journal.pone.0170545

Single Dose Caffeine Protects the Neonatal Mouse Brain against Hypoxia Ischemia

PLoS One. 2017 Jan 27;12(1):e0170545. doi: 10.1371/journal.pone.0170545. eCollection 2017.

Authors

Max Winerdal¹, Vijay Urmaliya², Malin E Winerdal³, Bertil B Fredholm², Ola Winqvist³, Ulrika Ådén¹

Affiliations

¹ Department of Women´s and Children´s Health, Karolinska Institutet, Stockholm, Sweden.
² Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
³ Department of Medicine, Karolinska Institutet, Stockholm, Sweden.

Abstract

In this randomized blinded study, we investigated caffeine 5 mg/kg treatment given directly after neonatal brain hypoxia ischemia. Brain morphology, behavior and key brain infiltrating immune populations were examined. Caffeine treatment significantly improves outcome when compared to phosphate buffered saline. Flow cytometric analysis of immune responses revealed no persistent immunological alterations. Given its safety caffeine emerges as a candidate for neuroprotective intervention after neonatal brain injury.

MeSH terms

Animals
Animals, Newborn
Caffeine / administration & dosage*
Disease Models, Animal
Dose-Response Relationship, Drug
Humans
Hypoxia-Ischemia, Brain / drug therapy*
Hypoxia-Ischemia, Brain / pathology
Mice
Neurons / drug effects*
Neurons / pathology
Neuroprotective Agents / administration & dosage*
Treatment Outcome

Substances

Neuroprotective Agents
Caffeine

Grants and funding

This work was supported by Medicinska Forskningsrådet (SE) regional agreement on medical training and clinical research, Marianne and Marcus Wallenberg foundation, Swedish Brain Foundation and Swedish Order of Freemasons in Stockholm. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.