Preparation and Characterization of Hyaluronic Acid-Polycaprolactone Copolymer Micelles for the Drug Delivery of Radioactive Iodine-131 Labeled Lipiodol

Shih-Cheng Chen; Ming-Hui Yang; Tze-Wen Chung; Ting-Syuan Jhuang; Jean-Dean Yang; Ko-Chin Chen; Wan-Jou Chen; Ying-Fong Huang; Shiang-Bin Jong; Wan-Chi Tsai; Po-Chiao Lin; Yu-Chang Tyan

doi:10.1155/2017/4051763

Preparation and Characterization of Hyaluronic Acid-Polycaprolactone Copolymer Micelles for the Drug Delivery of Radioactive Iodine-131 Labeled Lipiodol

Biomed Res Int. 2017:2017:4051763. doi: 10.1155/2017/4051763. Epub 2017 Jan 3.

Authors

Affiliations

¹ Office of Research and Development, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
² Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
³ Department of Biomedical Engineering, National Yang-Ming University, Taipei 112, Taiwan.
⁴ Invasive Cardiac Laboratory, Tan Tock Seng Hospital, Singapore 308433.
⁵ Department of Chemistry, Chung Yuan Christian University, Taoyuan 320, Taiwan.
⁶ Department of Pathology, Changhua Christian Hospital, Changhua 500, Taiwan.
⁷ Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
⁸ Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Nuclear Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
⁹ Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
¹⁰ Department of Chemistry, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
¹¹ Office of Research and Development, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 804, Taiwan; Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.

Abstract

Micelles, with the structure of amphiphilic molecules including a hydrophilic head and a hydrophobic tail, are recently developed as nanocarriers for the delivery of drugs with poor solubility. In addition, micelles have shown many advantages, such as enhanced permeation and retention (EPR) effects, prolonged circulation times, and increased endocytosis through surface modification. In this study, we measured the critical micelle concentrations, diameters, stability, and cytotoxicity and the cell uptake of micelles against hepatic cells with two kinds of hydrophilic materials: PEG-PCL and HA-g-PCL. We used ¹³¹I as a radioactive tracer to evaluate the stability, drug delivery, and cell uptake activity of the micelles. The results showed that HA-g-PCL micelles exhibited higher drug encapsulation efficiency and stability in aqueous solutions. In addition, the ¹³¹I-lipiodol loaded HA-g-PCL micelles had better affinity and higher cytotoxicity compared to HepG2 cells.

MeSH terms

Cell Line
Cell Survival / drug effects
Cell Survival / radiation effects
Contrast Media / administration & dosage
Contrast Media / pharmacokinetics
Contrast Media / toxicity
Drug Carriers / chemistry
Drug Delivery Systems*
Drug Stability
Ethiodized Oil / administration & dosage*
Ethiodized Oil / pharmacokinetics
Ethiodized Oil / toxicity
Hep G2 Cells
Hepatocytes / drug effects
Hepatocytes / metabolism
Hepatocytes / radiation effects
Humans
Hyaluronic Acid / analogs & derivatives
Hydrophobic and Hydrophilic Interactions
Iodine Radioisotopes / administration & dosage*
Iodine Radioisotopes / pharmacokinetics
Iodine Radioisotopes / toxicity
Micelles
Particle Size
Polyesters
Radiopharmaceuticals / administration & dosage*
Radiopharmaceuticals / pharmacokinetics
Radiopharmaceuticals / toxicity
Solubility

Substances

Contrast Media
Drug Carriers
Iodine Radioisotopes
Micelles
Polyesters
Radiopharmaceuticals
hyaluronic acid-polyethyleneglycol-polycaprolactone copolymer
polycaprolactone
Ethiodized Oil
Hyaluronic Acid