Data showing proliferation and differentiation of intestinal epithelial cells under targeted depletion of Notch ligands in mouse intestine

Toru Nakata; Hiromichi Shimizu; Sayaka Nagata; Go Ito; Satoru Fujii; Kohei Suzuki; Ami Kawamoto; Fumiaki Ishibashi; Reiko Kuno; Sho Anzai; Tatsuro Murano; Tomohiro Mizutani; Shigeru Oshima; Kiichiro Tsuchiya; Tetsuya Nakamura; Katsuto Hozumi; Mamoru Watanabe; Ryuichi Okamoto

doi:10.1016/j.dib.2016.12.045

Data showing proliferation and differentiation of intestinal epithelial cells under targeted depletion of Notch ligands in mouse intestine

Data Brief. 2016 Dec 29:10:551-556. doi: 10.1016/j.dib.2016.12.045. eCollection 2017 Feb.

Authors

Toru Nakata¹, Hiromichi Shimizu², Sayaka Nagata¹, Go Ito³, Satoru Fujii¹, Kohei Suzuki¹, Ami Kawamoto¹, Fumiaki Ishibashi¹, Reiko Kuno¹, Sho Anzai¹, Tatsuro Murano¹, Tomohiro Mizutani¹, Shigeru Oshima¹, Kiichiro Tsuchiya¹, Tetsuya Nakamura⁴, Katsuto Hozumi⁵, Mamoru Watanabe¹, Ryuichi Okamoto⁶

Affiliations

¹ Department of Gastroenterology and Hepatology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
² Department of Gastroenterology and Hepatology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan; Department of Medicine, University of California, San Francisco, San Francisco, CA, USA.
³ Department of Gastroenterology and Hepatology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan; Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel, D-24105 Kiel, Germany.
⁴ Department of Advanced Therapeutics in GI Diseases, Tokyo Medical and Dental University, Tokyo, Japan.
⁵ Department of Immunology, Tokai University School of Medicine, Isehara, Japan.
⁶ Department of Gastroenterology and Hepatology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan; Center for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University, Tokyo, Japan.

Abstract

The data on the immunohistochemical analysis of conditional Notch ligand knockout mice is presented. Targeted deletion of Jag1, Dll1, Dll4, or Dll1 plus Dll4 in Lgr5^+ve cells was induced by a Cre-mediated gene recombination, and differentiation or proliferation of the intestinal epithelial cells was examined by immunohistochemistry. These data are the extension of the data presented and discussed in the paper entitled "Indispensable role of non-canonical Notch signaling in the proliferation of Apc-deficient intestinal tumors" (Nakata et al., Submitted for publication) [1].