Isolation of Immune Cells for Adoptive Transfer

Methods Mol Biol. 2017:1527:321-344. doi: 10.1007/978-1-4939-6625-7_25.

Abstract

Adoptive transfer of T lymphocytes is a useful technique to characterize the role of the immune system in hypertension and vascular disease. Here we describe as an example the isolation of splenic T regulatory cells from donor mice processed to obtain a single cell suspension, followed by negative and positive selection to obtain CD4+ T cells and CD4+CD25+ Treg cells, respectively. Treg cells can be subsequently transferred to recipient animals.

Keywords: Adoptive transfer; CD4+CD25+ Treg cells; Flow cytometry; Immunofluorescence; Intravenous injection; Magnetic beads; T Cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer / methods*
  • Animals
  • CD4-Positive T-Lymphocytes / cytology
  • CD4-Positive T-Lymphocytes / metabolism
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Injections, Intravenous
  • Interleukin-2 Receptor alpha Subunit / metabolism
  • Mice
  • T-Lymphocytes, Regulatory / immunology
  • T-Lymphocytes, Regulatory / metabolism*

Substances

  • IL2RA protein, human
  • Interleukin-2 Receptor alpha Subunit