Abstract
Trimethyllysine hydroxylase (TMLH) catalyses C-3 hydroxylation of Nε-trimethyllysine in the first step of carnitine biosynthesis in humans. Studies on TMLH have been hampered by the lack of established chemical methods. We report that an Nε-trimethyllysine analogue that contains the fluoromethyl group can be used as a 1H and 19F NMR probe for studies on TMLH catalysis.
MeSH terms
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Biocatalysis
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Fluorine
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Halogenation
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Humans
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Lysine / analogs & derivatives*
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Lysine / biosynthesis
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Lysine / chemistry
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Magnetic Resonance Spectroscopy
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Mixed Function Oxygenases / metabolism*
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Molecular Probes / biosynthesis*
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Molecular Probes / chemistry
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Molecular Structure
Substances
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Molecular Probes
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Fluorine
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trimethyllysine
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Mixed Function Oxygenases
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trimethyl-lysine hydroxylase
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Lysine