Genome scan linkage analysis identifies a major quantitative trait loci for fatty acid composition in longissimus dorsi muscle in an F2 intercross between Landrace and Korean native pigs

Hee-Bok Park; Sang-Hyun Han; Chae-Kyoung Yoo; Jae-Bong Lee; Ji-Hyang Kim; Kwang-Soo Baek; Jun-Kyu Son; Sang-Min Shin; Hyun-Tae Lim; In-Cheol Cho

doi:10.5713/ajas.16.0562

Genome scan linkage analysis identifies a major quantitative trait loci for fatty acid composition in longissimus dorsi muscle in an F₂ intercross between Landrace and Korean native pigs

Asian-Australas J Anim Sci. 2017 Aug;30(8):1061-1065. doi: 10.5713/ajas.16.0562. Epub 2017 Jan 2.

Authors

Affiliations

¹ Subtropical Livestock Research Institute, National Institute of Animal Science, Rural Development Administration, Jeju 63242, Korea.
² Educational Science Research Institute, Jeju National University, Jeju 63243, Korea.
³ Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju 52828, Korea.
⁴ Korea Zoonosis Research Institute, Chonbuk National University, Iksan 54531, Korea.
⁵ Department of Animal Science, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 52828, Korea.

Abstract

Objective: This study was conducted to locate quantitative trait loci (QTL) influencing fatty acid (FA) composition in a large F₂ intercross between Landrace and Korean native pigs.

Methods: Eighteen FA composition traits were measured in more than 960 F₂ progeny. All experimental animals were genotyped with 165 microsatellite markers located throughout the pig autosomes.

Results: We detected 112 QTLs for the FA composition; Forty seven QTLs reached the genome-wide significant threshold. In particular, we identified a cluster of highly significant QTLs for FA composition on SSC12. QTL for polyunsaturated fatty acid on pig chromosome 12 (F-value = 97.2 under additive and dominance model, nominal p-value 3.6×10^-39) accounted for 16.9% of phenotypic variance. In addition, four more QTLs for C18:1, C18:2, C20:4, and monounsaturated fatty acids on the similar position explained more than 10% of phenotypic variance.

Conclusion: Our findings of a major QTL for FA composition presented here could provide helpful information to locate causative variants to improve meat quality traits in pigs.

Keywords: Fatty Acid; Genome-wide Linkage Analysis; Korean Native Pigs; Landrace; Quantitative Trait Locus.