Neuroprotective effect of Spilanthes acmella Murr. on pesticide-induced neuronal cells death

Wilasinee Suwanjang; Bongkot Khongniam; Sujittra Srisung; Supaluk Prachayasittikul; Virapong Prachayasittikul

doi:10.1016/j.apjtm.2016.11.012

Neuroprotective effect of Spilanthes acmella Murr. on pesticide-induced neuronal cells death

Asian Pac J Trop Med. 2017 Jan;10(1):35-41. doi: 10.1016/j.apjtm.2016.11.012. Epub 2016 Dec 27.

Authors

Wilasinee Suwanjang¹, Bongkot Khongniam¹, Sujittra Srisung², Supaluk Prachayasittikul³, Virapong Prachayasittikul⁴

Affiliations

¹ Center for Research and Innovation, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand.
² Department of Chemistry, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand.
³ Center of Data Mining and Biomedical Informatics, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand.
⁴ Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand. Electronic address: virapong.pra@mahidol.ac.th.

PMID: 28107862
DOI: 10.1016/j.apjtm.2016.11.012

Abstract

Objective: To investigate protective effects of Spilanthes acmella (S. acmella) Murr. extracts against pesticide-induced neuronal cells death and to elucidate the underlying molecular mechanism in dopaminergic (SH-SY5Y) cells lines.

Methods: Cell viability of SH-SY5Y cells was studied by treating the cells with various concentration of pirimicarb for 24 h. Neuroprotective effect of S. acmella Murr. extracts was investigated by adding the plant extracts to the medium for 24 h prior to the incubation with 100 μM H₂O₂ or with pirimicarb for 24 h. Control-untreated cells were incubated with the culture medium. Cell viability was measured by MTT assay, calpain and calpastatin expressions were analyzed by Western blotting and immunocytochemistry.

Results: Pretreatment of SH-SY5Y cells with S. acmella Murr. extracts (1 μg/mL) for 24 h significantly increased the dopaminergic neurons in pirimicarb-induced neurotoxicity. In addition, pretreatment with the S. acmella Murr. extracts led to decreased calpain but increased calpastatin protein levels.

Conclusion: S. acmella Murr. extracts exerted neuroprotective effect, via an alteration of calcium homeostasis, against pirimicarb induced neurotoxicity. The S. acmella Murr. might be a potential natural candidate with neuroprotective activity.

Keywords: Calpain; Calpastatin; Neuronal cell; Pesticide; Pirimicarb; Spilanthes acmella Murr..