Decatamariic acid, a new mitochondrial respiration inhibitor discovered by pesticidal screening using drug-sensitive Saccharomyces cerevisiae

Yoshihiro Watanabe; Takuya Suga; Satomi Narusawa; Masato Iwatsuki; Kenichi Nonaka; Takuji Nakashima; Yasuo Shinohara; Takahiro Shiotsuki; Naoya Ichimaru; Hideto Miyoshi; Yukihiro Asami; Satoshi Ōmura; Kazuro Shiomi

doi:10.1038/ja.2016.164

Decatamariic acid, a new mitochondrial respiration inhibitor discovered by pesticidal screening using drug-sensitive Saccharomyces cerevisiae

J Antibiot (Tokyo). 2017 Apr;70(4):395-399. doi: 10.1038/ja.2016.164. Epub 2017 Jan 18.

Authors

Yoshihiro Watanabe¹, Takuya Suga², Satomi Narusawa², Masato Iwatsuki^{1

2}, Kenichi Nonaka^{1

2}, Takuji Nakashima^{1

2}, Yasuo Shinohara³, Takahiro Shiotsuki⁴, Naoya Ichimaru⁵, Hideto Miyoshi⁶, Yukihiro Asami^{1

2}, Satoshi Ōmura², Kazuro Shiomi^{1

2}

Affiliations

¹ Department of Drug Discovery Sciences, Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.
² Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
³ Institute for Genome Research, Tokushima University, Tokushima, Japan.
⁴ Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Ibaraki, Japan.
⁵ Faculty of Bioenvironmental Science, Kyoto Gakuen University, Kyoto, Japan.
⁶ Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.

PMID: 28096549
DOI: 10.1038/ja.2016.164

Abstract

A new decalin, decatamariic acid, was isolated from a cultured broth of the fungus Aspergillus tamarii FKI-6817. Its absolute configuration was elucidated by NMR and electronic circular dichroism. Decatamariic acid (10 μM) elicited ~50% inhibition of the ATP production in mitochondria isolated from wild-type Saccharomyces cerevisiae without affecting the activities of respiratory enzymes. The action manner of this compound may be interesting as a possible seed for new pesticides.

MeSH terms

Adenosine Triphosphate / antagonists & inhibitors
Adenosine Triphosphate / biosynthesis
Aspergillus / chemistry
Aspergillus / classification
Drug Evaluation, Preclinical
Fermentation
Magnetic Resonance Spectroscopy
Mitochondria / drug effects
Mitochondria / metabolism*
Molecular Conformation
Naphthalenes / chemistry*
Naphthalenes / pharmacology*
Oxygen Consumption / drug effects*
Pesticides / chemistry
Pesticides / pharmacology*
Saccharomyces cerevisiae / drug effects*

Substances

Naphthalenes
Pesticides
decatamariic acid
Adenosine Triphosphate